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Protein kinase C-β and -δ isoenzymes promote arachidonic acid production and proliferation of MonoMac-6 cells

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Abstract

In this study, we investigated the putative roles of certain protein kinase C (PKC) isoenzymes in the regulation of proliferation and arachidonic acid (AA) release in the human monocytoid MonoMac-6 cell line. Experiments employing specific PKC inhibitors and molecular biological methods (RNA-interference, recombinant overexpression) revealed that the two dominantly expressed isozymes, i.e., the “conventional” cPKCβ and the “novel” nPKCδ, promote AA production and cellular proliferation. In addition, using different phospholipase A2 (PLA2) inhibitors, we were able to show that the calcium-independent iPLA2 as well as diacylglycerol lipase (but not the cytosolic PLA2) function as “downstream” targets of cPKCβ and nPKCδ. In addition, we have also found that, among the other existing PKC isoforms, cPKCα plays a minor inhibitory role, whereas nPKCɛ and aPKCζ apparently do not regulate these cellular processes. In conclusion, in this paper we provide the first evidence that certain PKC isoforms play pivotal, specific, and (at least partly) antagonistic roles in the regulation of AA production and cellular proliferation of human monocytoid MonoMac-6 cells.

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Acknowledgments

This work was supported by Hungarian Research grants: OTKA T37531, OTKA T049231, OTKA K63153, NKFP 1A/008/04, RET 06/2004. The authors declare no competing financial interests.

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Authors and Affiliations

  1. Department of Physiology, University of Debrecen, Research Center for Molecular Medicine, Medical and Health Science Center, Nagyerdei krt. 98., 4032, Debrecen, Hungary

    Zoltán Griger, Edit Páyer, Balázs I. Tóth, László Kovács & Tamás Bíró

  2. Cell Physiology Research Group of the Hungarian Academy of Sciences, University of Debrecen, Research Center for Molecular Medicine, Medical and Health Science Center, Debrecen, Hungary

    Balázs I. Tóth, László Kovács & Tamás Bíró

  3. IIIrd Department of Internal Medicine, University of Debrecen, Research Center for Molecular Medicine, Medical and Health Science Center, Debrecen, Hungary

    Zoltán Griger, Ildikó Kovács & Sándor Sipka

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  1. Zoltán Griger
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  2. Edit Páyer
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  3. Ildikó Kovács
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  4. Balázs I. Tóth
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  5. László Kovács
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Correspondence to Tamás Bíró.

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Griger, Z., Páyer, E., Kovács, I. et al. Protein kinase C-β and -δ isoenzymes promote arachidonic acid production and proliferation of MonoMac-6 cells. J Mol Med 85, 1031–1042 (2007). https://doi.org/10.1007/s00109-007-0209-y

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  • DOI: https://doi.org/10.1007/s00109-007-0209-y

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