Abstract
A reverse-phase liquid chromatographic method has been established for analysis of oroxylin A, chrysin, baicalein, and hispidulin in the roots of Oroxylum indicum (Bignoneaceae). Under the optimized conditions, well-resolved separation of pure compounds with significantly different R t values was achieved on chromolith C18 column (100 mm × 4.6 mm, 10 μm) using isocratic elution of acetonitrile and water (0.1 % trifluoroacetic acid); with acceptable validation results such as linearity, sensitivity, and recovery in terms of %RSD. The calibration curves were linear in the concentration range of 200–500 ng ml−1. Method was validated as per ICH guidelines. The developed reproducible and robust method may be applied for the quality assays of O. indicum and phytopreparations.
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Acknowledgments
We are thankful to Director, Central Institute of Medicinal and Aromatic Plants, Lucknow for providing fundamental facilities for the research. One of the author AKY is thankful to Council of Scientific and Industrial Research (CSIR) for the award of Fellowship.
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Yadav, A.K., Manika, N., Bagchi, G.D. et al. Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC. Med Chem Res 22, 2222–2227 (2013). https://doi.org/10.1007/s00044-012-0214-8
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DOI: https://doi.org/10.1007/s00044-012-0214-8