Skip to main content
SpringerLink
Log in

Evaluation of biological effects of the naproxen

  • Original Research
  • Published:
Medicinal Chemistry Research Aims and scope Submit manuscript

Abstract

This work aims to evaluate biological effects of naproxen through of the labeling of blood constituents with technetium-99m (99mTc), survival of bacterial cultures and electrophoresis profile of plasmid DNA. Blood samples from Wistar rats were incubated with naproxen or with saline (0.9% NaCl) as control, and radiolabeling of blood constituents was performed. Influence of naproxen on the E. coli AB1157 culture growth and survival in the presence or absence of SnCl2 was used to assess cytotoxic effect. Electrophoretic profile in agarose gels of bacterial plasmids in presence or absence of SnCl2 was used to evaluate antioxidant and genotoxic potential of naproxen. Results obtained suggest that naproxen could not interfere on the labeling of blood constituents with 99mTc, but it could present cytotoxic effects and protect E. coli cultures from the lethal effect of stannous chloride at low concentrations. Moreover, naproxen could present genotoxic effect in isolated plasmid DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abramson SB, Amin AR, Clancy R, Attur M (2001) Nitric oxide and inflammatory mediators in the osteoarthritis. Curr Rheumatol Rep 6:535–544

    Article  Google Scholar 

  • Abreu PRC, Almeida MC, Bernardo RM, Bernardo LC, Brito LC, Garcia EAC, Fonseca AS, Bernardo-Filho M (2006) Guava extract (Psidium gaujava) alters the labeling of blood constituents with technetium-99 m. J Zheijiang Univ Sci B 7:429–435

    Article  CAS  Google Scholar 

  • Agostinho RT, Santos-Filho SD, Fonseca AS, Missailidis S, Bernardo-Filho M (2008) The effect of an extract from ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99 m and on the survival of Escherichia coli. Brazilian Archives of Biology and Techonology 51:157–162

    Article  Google Scholar 

  • Almeida MC, Soares SF, Abreu PR, Jesus LM, Brito LC, Bernardo-Filho M (2007) Protective effect of an aqueous extract of Harpagophytum upon Escherichia coli strains submitted to the lethal action of stannous chloride. Cell Mol Biol (Noisy-le-grand-France) 53:923–992

    Google Scholar 

  • Assis ML, De Mattos JC, Caceres MR, Dantas FJ, Asad LM, Asad NR, Bezerra RJ, Caldeira-de-Araújo A, Bernardo-Filho M (2002) Adaptive response to H2O2 protects against SnCl2 damage: the OxyR system involvement. Biochimie 84:291–294

    Article  PubMed  CAS  Google Scholar 

  • Benarroz MO, Fonseca AS, Rocha GS, Frydman JN, Rocha VC, Pereira MO, Bernardo-Filho M (2008) Cinnamomum zeylanicum extract on the radiolabelling of blood constituents and the morphometry of red blood cells: In vitro assay. Appl Radiat Isot 66:139–146

    Article  PubMed  CAS  Google Scholar 

  • Bernardo-Filho M, Moura INS, Boasquevisque M (1983) 99m technetium-labeled red blood “in vitro”. Braz Arch Biol Techonol 4:455–461

    Google Scholar 

  • Bernardo-Filho M, Cunha MC, Valsa JO, Araujo AC, Silva FC, Fonseca AS (1994) Evaluation of potential genotoxicity of stannous chloride: inactivation, filamentation and lysogenic induction of Escherichia coli. Food Chem Toxicol 32:477–479

    Article  PubMed  CAS  Google Scholar 

  • Callahan RJ, Rabito CA (1990) Radiolabeling of erythrocytes with technetium-99m: role of band-3 protein in the transport of pertechnetate across the cell membrane. J Nucl Med 31:2004–2008

    PubMed  CAS  Google Scholar 

  • Costa D, Moutinho L, Lima JLF, Fernandes E (2006) Antioxidant activity and inhibithion of human neutrophil oxidative burst mediated by arylpropionic acid non-steroidal anti-inflamatory drugs. Biol Pharm Bull 29:1659–1670

    Article  PubMed  CAS  Google Scholar 

  • Dantas FJS, Moraes MO, Carvalho EF, Valsa JO, Bernardo-Filho M, Caldeira-de-Araújo A (1996) Lethality induced by stannous chloride on Escherichia coli AB1157: participation of reactive oxygen species. Food Chem Toxicol 34:959–962

    Article  PubMed  CAS  Google Scholar 

  • Dantas FJS, Moraes O, Mattos CPJ, Bezerra JACR, Carvalho FE, Bernardo-Filho M, Araújo CA (1999) Stannous chloride mediates single strand breaks in plasmid DNA through reactive oxygen species formation. Toxicol Lett 110:129–136

    Article  PubMed  CAS  Google Scholar 

  • Dantas FJS, De Mattos JCP, Viana ME, Lage CAS, Cabral-Neto JB, Leitão AC, Bernardo-Filho M, Bezerra RJAC, Carvalho JJ, Caldeira-de-Araújo A (2002) Genotoxic effects of stannous chloride (SnCl2) in K562 cell line. Food Chem Toxicol 40:1493–1498

    Article  PubMed  CAS  Google Scholar 

  • de Mattos JC, Dantas FJ, Bezerra RJ, Bernardo-Filho M, Cabral-Neto JB, Lage C, Leitão AC, Caldeira-de-Araújo A (2000) Damage induced by stannous chloride in plasmid DNA. Toxicol Lett 116:159–163

    Article  PubMed  Google Scholar 

  • Fendrick AM, Greenberg BP (2009) A review of the benefits and risks of nonsteroidal anti-inflammatory drugs in the management of mild-to-moderate osteoarthritis. Osteopathic Med Primary Care 6:3

    Google Scholar 

  • Ferreira-Machado SC, Rodrigues MP, Nunes AP, Dantas FJ, De Mattos JC, Silva CR, Moura EG, Bezerra RJ, Caldeira-de-Araujo A (2004) Genotoxic potentiality of aqueous extract prepared from Chrysobalanus icaco L. leaves. Toxicol Lett 151:481–488

    Article  PubMed  CAS  Google Scholar 

  • Fitzgerald GA, Patrono C (2001) The coxibs, selective inhibitor of cyclooxygenase-2. N Engl J Med 345:433–444

    Article  PubMed  CAS  Google Scholar 

  • Fonseca AS, Frydman JN, Rocha VC, Bernardo-Filho M (2007) Acetylsalicylic acid decreases the labeling of blood constituents with technetium-99M. Acta Biol Hung 2:187–198

    Article  Google Scholar 

  • Frydman JNG, Rocha VC, Benarroz MO, Rocha GS, Pereira MO, Fonseca AS, Bernardo-Filho M (2008) Assessment of effects of a Cordia salicifolia extract on the radiolabeling of blood constituents and on the morphology of red blood cells. J Med Food 11:767–772

    Article  PubMed  CAS  Google Scholar 

  • Hassan HN, Barsoum BN, Habid IH (1999) Simultaneous spectrophotometric determination of rutin, quercetin and ascorbic acid in drugs using a Kalman Filter approach. J Pharm Biomed Anal 20:315–320

    Article  PubMed  CAS  Google Scholar 

  • Insel PA (2001) Analgesic-antipyretics and antiinflammatory agents and drugs employed in the treatment of gout. In: Harman JG, Limbird LE, Gilman AG (eds) The pharmacological basis of therapeutics, 10th edn. McGraw/Hill, New York, pp 617–657

    Google Scholar 

  • Luria SE, Burrous JW (1957) Hybridization between E.coli and Shigella. J Bacteriol 74:461–476

    PubMed  CAS  Google Scholar 

  • Melo SF, Soares SF, da Costa RF, da Silva CR, de Oliveira MB, Bezerra RJ, Caldeira-de-Araujo A, Bernardo-Filho M (2001) Effect of the Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts against the stannous chloride oxidative damage in Escherichia coli. Mutat Res 496:33–38

    PubMed  CAS  Google Scholar 

  • Oh TY, Ahn GJ, Choi SM, Ahn BO, Kim WB (2005) Increased susceptibility of ethanol-treated gastric mucosa to naproxen and its inhibition by DA-9601, an Artemisia asiatica extract. World J Gastroenterol 11:7450–7456

    PubMed  CAS  Google Scholar 

  • Orhan H, Sahin G (2001) In vitro effects of NSAIDS and paracetamol on oxidative stress-related parameters of human erythrocytes. Exp Toxicol Pathol 53:133–140

    Article  PubMed  CAS  Google Scholar 

  • Pereira MO, Rocha GS, Lombardi SS, Geller M, Pereira MJ, Santos-Filho SD, Fonseca AS, Bernardo-Filho M (2008) Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid. Braz Arch Biol Technol 51:135–141

    Article  Google Scholar 

  • Presta GA, Fonseca AS, Bernardo-Filho M (2007) A Chrysobalanus icaco extract alters the plasmid topology and the effects of stannous chloride on the DNA of plasmids. Braz J Pharmacogn 17:331–335

    Google Scholar 

  • Pungartnik C, Viau C, Picada J, Caldeira-de-Araujo A, Henriques JA, Brendel M (2005) Genotoxicity of stannous chloride in yeast and bacteria. Mutat Res 583:146–157

    PubMed  CAS  Google Scholar 

  • Ricchi P, Di Matola T, Ruggiero G (2002) Effect of non-steroidal anti-inflammatory drugs on colon carcinoma Caco-2 cell responsiveness to topoisomerase inhibitor drugs. Br J Cancer 86:1501–1509

    Article  PubMed  CAS  Google Scholar 

  • Saha GB (2004) Fundamentals in nuclear pharmacy. Springer-Verlag, New York

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Extraction and purification of plasmid DNA. In: Molecular cloning. A laboratory manual. Cold Spring Harbour Laboratory Press, New York

  • Schiff M, Minic M (2004) Comparison of the analgesic efficacy and safety of nonprescription doses of naproxen sodium and ibuprofen in the treatment of osteoarthritis the knee. J Rheumatol 31:1373–1383

    PubMed  CAS  Google Scholar 

  • Van Tunder MW, Sholten RJ, Koes BW, Deyo RA (2000) Nonsteroidal anti-inflammatory drugs for low back pain: a systematic review within the framework of the Cochrane Collaboration Back Review Group. Spine 25:2501–2513

    Article  Google Scholar 

  • Warner TD, Vojnovic I, Bishop-Bailey D, Mitchell JA (2006) Influence of plasma protein on the potencies of inhibitors of cyclooxygenase-1 and-2. Faseb J 20:542–544

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by grants and financial support by the Universidade Federal do Rio Grande no Norte (UFRN), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Carlos Chaga Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and the Universidade do Estado do Rio de Janeiro (UERJ).

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências da Saúde, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Avenida General Gustavo Cordeiro de Farias, s/n, Natal, 59010180, Brazil

    Marcia de Oliveira Pereira, Gabrielle de Souza Rocha & Aldo Cunha Medeiros

  2. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, Vila Isabel, Rio de Janeiro, 20551030, Brazil

    Marcia de Oliveira Pereira, Gabrielle de Souza Rocha, Adenilson de Souza da Fonseca & Mario Bernardo-Filho

  3. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro, 20211040, Brazil

    Adenilson de Souza da Fonseca

  4. Centro de Ciências da Saúde, Centro Universitário Serra dos Órgãos, Avenida Alberto Torres, 111, Alto, Teresópolis, RJ, 25964004, Brazil

    Adenilson de Souza da Fonseca

  5. Instituto Nacional do Câncer, Praça Cruz Vermelha, 23, 20230130, Rio de Janeiro, Brazil

    Mario Bernardo-Filho

Authors
  1. Marcia de Oliveira Pereira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gabrielle de Souza Rocha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aldo Cunha Medeiros
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Adenilson de Souza da Fonseca
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mario Bernardo-Filho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adenilson de Souza da Fonseca.

Rights and permissions

Reprints and permissions

About this article

Cite this article

de Oliveira Pereira, M., de Souza Rocha, G., Medeiros, A.C. et al. Evaluation of biological effects of the naproxen. Med Chem Res 21, 1433–1438 (2012). https://doi.org/10.1007/s00044-011-9665-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00044-011-9665-6

Keywords

Search

Navigation