Abstract
Ultrasonically assisted extraction and Soxhlet extraction using 95% ethanol were performed to obtain ultrasonically assisted extract of Agrimonia pilosa (UEA) and Soxhlet extract of Agrimonia pilosa (SEA). The effects of the different extracting methods on the total phenolic (TP) and total flavonoid (TF) content of the extracts from Agrimonia pilosa were investigated. The findings showed that UEA contained higher TP and TF content than SEA. To understand better the effect of ultrasonic extraction, the antioxidant activities of UEA and SEA were evaluated with different antioxidant testing systems in vitro to determine reducing power, total antioxidant capacity, and free radical scavenging assays, including 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Both SEA and UEA exhibited satisfied antioxidant activity superior to that of synthetic antioxidant butylated hydroxytoluene (BHT). The antioxidant activity of the tested subjects was in the order of UEA > SEA > BHT. Their high phenolic and flavonoid contents could be responsible for their antioxidant activity and pharmacologic actions.
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Acknowledgments
This study was supported by the 973 project (2007CB516805), the Guangxi Province Science Foundation (0832024 and 0832096), Guangxi’s Medicine Talented Persons Small Highland Foundation (0808), and the Innovation Project of Guangxi Graduate Education (2007106020703M59).
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He, C., Ji, X., Pan, Y. et al. Antioxidant activity of alcoholic extract of Agrimonia pilosa Ledeb. Med Chem Res 19, 448–461 (2010). https://doi.org/10.1007/s00044-009-9201-0
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DOI: https://doi.org/10.1007/s00044-009-9201-0