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Effect of queen excluders on ovary activation in workers of the Eastern honeybee Apis cerana

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Abstract

Honeybee workers generally refrain from personal reproduction when a queen is present. Workers discern the presence and fecundity of the queen via volatile pheromones that permeate throughout the colony. Pheromones are emitted both by the queen herself and by the brood that she produces. If pheromone production is disrupted, some workers initiate egg laying. The Eastern honeybee Apis cerana is unusual in that workers have high levels of ovary activation even in the presence of a queen. To investigate the effect of disruption to pheromone dispersal, we fitted three A. cerana colonies with vertical queen excluders, thus splitting the colonies into a half containing a queen and a half without a queen. We regularly sampled adult workers from both sides of the excluder for 3 weeks. We also sampled workers from three control colonies that did not contain excluders. We found a significant increase in worker ovary activation 3 days after addition of excluders, suggesting that the reduced dispersal of pheromones allowed some workers to become reproductively active. Workers attempted to rear queen cells on the queenless halves of all three colonies. Queen-rearing ceased on day 9, at which time no queen-laid brood remained on the queenless halves of the colonies. Ovary activation rates continued to climb until day 9 and then gradually began to decline. With the exception of one egg, we did not observe worker-laid brood on the queenless side of the colonies, suggesting that workers continued policing eggs laid by workers. We conclude that if the distribution of brood pheromone is impeded, workers prepare to supersede their queen, accompanied by high levels of worker ovary activation. However, because workers continue to police each other, high ovary activation does not result in worker-produced drones.

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Acknowledgments

We thank Zhongwen Hu for his assistance in the field. This research was supported by an Endeavour Research Fellowship (MJH) and Australian Research Council grants (MB and BPO).

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Authors and Affiliations

  1. Behaviour and Genetics of Social Insects Laboratory, School of Biological Sciences, University of Sydney, Macleay Building A12, Sydney, NSW, 2006, Australia

    M. J. Holmes, B. P. Oldroyd & M. Beekman

  2. Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Science, Kunming, Yunnan, People’s Republic of China

    K. Tan

  3. Eastern Bee Research Institute of Yunnan Agricultural University, Heilongtan, Kunming, People’s Republic of China

    K. Tan & Z. Wang

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  1. M. J. Holmes
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  2. K. Tan
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  4. B. P. Oldroyd
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Correspondence to M. J. Holmes.

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Holmes, M.J., Tan, K., Wang, Z. et al. Effect of queen excluders on ovary activation in workers of the Eastern honeybee Apis cerana . Insect. Soc. 61, 191–196 (2014). https://doi.org/10.1007/s00040-014-0344-8

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