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Cytoplasmic dynein and early endosome transport

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Abstract

Microtubule-based distribution of organelles/vesicles is crucial for the function of many types of eukaryotic cells and the molecular motor cytoplasmic dynein is required for transporting a variety of cellular cargos toward the microtubule minus ends. Early endosomes represent a major cargo of dynein in filamentous fungi, and dynein regulators such as LIS1 and the dynactin complex are both required for early endosome movement. In fungal hyphae, kinesin-3 and dynein drive bi-directional movements of early endosomes. Dynein accumulates at microtubule plus ends; this accumulation depends on kinesin-1 and dynactin, and it is important for early endosome movements towards the microtubule minus ends. The physical interaction between dynein and early endosome requires the dynactin complex, and in particular, its p25 component. The FTS-Hook-FHIP (FHF) complex links dynein–dynactin to early endosomes, and within the FHF complex, Hook interacts with dynein–dynactin, and Hook-early endosome interaction depends on FHIP and FTS.

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Acknowledgments

We thank Dr. Samara L. Reck-Peterson for critical reading and very helpful comments. The authors’ work was supported by the National Institutes of Health grant RO1 GM097580 (to X. X.), a Uniformed Services University intramural grant BIO-71-1972 (to X. X.), the Biotechnology and Biological Sciences Research Council grant BB/F01189X/1 (to H. N. A. and Elaine Bignell), the Wellcome Trust grant 084660/Z/08/Z (to H. N. A. and Joan Tilburn), the Spanish Government grant BIO2012-30695 (to M. A. P.) and Comunidad de Madrid grant S2012/BMD2414 (to M. A. P.).

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  1. Department of Biochemistry and Molecular Biology, F. Edward Hébert School of Medicine, The Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA

    Xin Xiang, Rongde Qiu, Xuanli Yao & Jun Zhang

  2. Microbiology Section, Department of Medicine, Imperial College London, London, SW7 2AZ, UK

    Herbert N. Arst Jr.

  3. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain

    Herbert N. Arst Jr. & Miguel A. Peñalva

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  1. Xin Xiang
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Xiang, X., Qiu, R., Yao, X. et al. Cytoplasmic dynein and early endosome transport. Cell. Mol. Life Sci. 72, 3267–3280 (2015). https://doi.org/10.1007/s00018-015-1926-y

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