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Insights into MHC class I antigen processing gained from large-scale analysis of class I ligands

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Abstract

Short peptides derived from intracellular proteins and presented on MHC class I molecules on the cell surface serve as a showcase for the immune system to detect pathogenic or malignant alterations inside the cell, and the sequencing and analysis of the presented peptide pool has received considerable attention over the last two decades. In this review, we give a comprehensive presentation of the methods employed for the large-scale qualitative and quantitative analysis of the MHC class I ligandome. Furthermore, we focus on insights gained into the underlying processing pathway, especially involving the roles of the proteasome, the TAP complex, and the peptide specificities and motifs of MHC molecules. The identification of post-translational modifications in MHC ligands and their implications for processing are also considered. Finally, we review the correlations of the ligandome to the proteome and the transcriptome.

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Authors and Affiliations

  1. Institute for Cell Biology, Department of Immunology, Eberhard Karls University Tübingen, Tübingen, Germany

    Gabor Mester, Vanessa Hoffmann & Stefan Stevanović

  2. Abteilung für Immunologie, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 15, 72076, Tübingen, Germany

    Stefan Stevanović

Authors
  1. Gabor Mester
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  2. Vanessa Hoffmann
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  3. Stefan Stevanović
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Correspondence to Stefan Stevanović.

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Mester, G., Hoffmann, V. & Stevanović, S. Insights into MHC class I antigen processing gained from large-scale analysis of class I ligands. Cell. Mol. Life Sci. 68, 1521–1532 (2011). https://doi.org/10.1007/s00018-011-0659-9

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  • DOI: https://doi.org/10.1007/s00018-011-0659-9

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