Skip to main content
SpringerLink
Log in

Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis

  • Research Article
  • Published:
Cellular and Molecular Life Sciences Aims and scope Submit manuscript

Abstract.

Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p–Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q6/DMQ6 ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ6 accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC and Centre for Biomedical Research on Rare Diseases (CIBERER), Carretera de Utrera, km 1, ISCIII, 41013, Sevilla, Spain

    S. Padilla, M. Jiménez-Hidalgo, J. M. López-Martín, A. Martín-Montalvo, P. Navas & C. Santos-Ocaña

  2. Department of Chemistry and Biochemistry, UCLA, Los Angeles, 90095, CA, USA

    U. C. Tran & C. F. Clarke

Authors
  1. S. Padilla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. U. C. Tran
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Jiménez-Hidalgo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. M. López-Martín
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Martín-Montalvo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. F. Clarke
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. P. Navas
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. C. Santos-Ocaña
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Santos-Ocaña.

Additional information

Received 04 September 2008; received after revision 22 October 2008; accepted 23 October 2008

Electronic supplementary material

Below are the electronic supplementary materials.

ESM (PDF 460 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Padilla, S., Tran, U.C., Jiménez-Hidalgo, M. et al. Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis. Cell. Mol. Life Sci. 66, 173 (2009). https://doi.org/10.1007/s00018-008-8547-7

Download citation

  • Published:

  • DOI: https://doi.org/10.1007/s00018-008-8547-7

Keywords.

Search

Navigation