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Simvastatin reduces VCAM-1 expression in human umbilical vein endothelial cells exposed to lipopolysaccharide

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Abstract

Objective

Reducing the expression of endothelial cell adhesion molecules (ECAMs) is known to decrease inflammation-induced vascular complications. In this paper we looked at whether statins can reduce inflammation-induced ECAM expression after lipopolysaccharide (LPS) treatment in endothelial cells.

Methods

Human umbilical vein endothelial cells (HUVECs) were pretreated with different concentrations of simvastatin, atorvastatin, and rosuvastatin and subsequently exposed to 5 μg/ml LPS. Semi-quantitative RT-PCR analysis was used to measure the mRNA expression of ECAMs, including VCAM-1, ICAM-1, and E-selectin.

Results

VCAM-1 mRNA appeared to be the only target that was affected by the statins, with its expression being partially and almost completely reduced by simvastatin at 50 and 125 μM concentrations, respectively, and only partially reduced by atorvastatin, but not reduced by rosuvastatin. VCAM-1 protein production was inhibited by simvastatin at concentrations from 5 to 125 μM. Leukocyte–endothelial cell adhesion assay revealed that simvastatin could inhibit the adhesion of labelled U937 cells to the HUVEC monolayer.

Conclusions

This study showed that simvastatin reduces VCAM-1 expression in HUVECs exposed to LPS and decreases leukocyte–endothelial cell adhesion.

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Abbreviations

ECAMs:

Endothelial cell adhesion molecules

VCAM-1:

Vascular cell adhesion molecule-1

ICAM-1:

Intercellular cell adhesion molecule-1

E-selectin:

Endothelial cell selectin

HUVECs:

Human umbilical vein endothelial cells

LPS:

Lipopolysaccharide

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Acknowledgments

The work was supported by the Chang Gung Memorial Hospital (CMRPG880511), Taiwan.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, No. 123, Ta-Pei Road, Niao-Sung District, Kaohsiung, Taiwan

    Johnson Chia-Shen Yang, Faye Huang, Chia-Jung Wu, Yi-Chun Chen, Tsu-Hsiang Lu & Ching-Hua Hsieh

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  1. Johnson Chia-Shen Yang
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  2. Faye Huang
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  3. Chia-Jung Wu
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  6. Ching-Hua Hsieh
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Corresponding author

Correspondence to Ching-Hua Hsieh.

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Responsible Editor: Makoto Katori.

J.C.-S. Yang and F. Huang contributed equally to the work as the first author.

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Yang, J.CS., Huang, F., Wu, CJ. et al. Simvastatin reduces VCAM-1 expression in human umbilical vein endothelial cells exposed to lipopolysaccharide. Inflamm. Res. 61, 485–491 (2012). https://doi.org/10.1007/s00011-012-0435-9

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  • DOI: https://doi.org/10.1007/s00011-012-0435-9

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