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Anti-inflammatory action of sulfated glucosamine on cytokine regulation in LPS-activated PMA-differentiated THP-1 macrophages

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Abstract

Objective

The aim of this study was to evaluate the effect of sulfated glucosamine (SGlc) on the regulation of inflammatory cytokines and profiles involved in immunological activities. Changes in the inflammatory profiles of lipopolysaccharide (LPS)-activated phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were investigated following SGlc treatment.

Methods

Human THP-1 macrophages were used to evaluate anti-inflammatory profiles. The cytokine secretion levels were measured by enzyme-linked immunosorbent assay (ELISA). Effects of SGlc on the regulation of mRNA and protein levels were determined using RT-PCR and Western blot analysis. The effect of SGlc on the activation of mitogen-activated protein kinases (MAPKs) and NF-κB protein was also determined by Western blot analysis.

Results

Treatment of THP-1 cells with SGlc inhibited the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. In addition, SGlc suppressed the mRNA and protein expression levels of inflammatory mediators such as inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, IL-1β, IL-6, 5-lipoxygenase and cytoplasmic phospholipase A2 in LPS-activated THP-1 macrophages. Furthermore, we confirmed that the LPS-activated transcriptions of MAPKs and NF-κB were inhibited by SGlc treatment in PMA-differentiated THP-1 macrophages.

Conclusion

These results suggest that SGlc can be considered as a potential anti-inflammatory supplement.

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References

  1. Wang Q, Xia M, Liu C, Guo H, Ye Q, Hu Y, Zhang Y, Hou M, Zhu H, Ma J, Ling W. Cyanidin-3-O-β-glucoside inhibits iNOS and COX-2 expression by inducing liver X receptor alpha activation in THP-1 macrophages. Life Sci. 2008;83:176–84.

    Article  PubMed  CAS  Google Scholar 

  2. Kishimoto Y, Tani M, Uto-Kondo H, Iizuka M, Saita E, Sone H, Kurata H, Kondo K. Astaxanthin suppresses scavenger receptor expression and matrix metalloproteinase activity in macrophages. Eur J Nutr. 2010;49:119–26.

    Article  PubMed  CAS  Google Scholar 

  3. Park EK, Jung HS, Yang HI, Yoo MC, Kim C, Kim KS. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm Res. 2007;56:45–50.

    Article  PubMed  CAS  Google Scholar 

  4. Di Giovine FS, Malawista SE, Thornton E, Duff GW. Urate crystals stimulate production of tumour necrosis factor alpha from human blood monocytes and synovial cells cytokine mRNA and protein kinetics, and cellular distribution. J Clin Invest. 1991;87:1375–81.

    Article  PubMed  CAS  Google Scholar 

  5. Coleman JW. Nitric oxide in immunity and inflammation. Int Immunopharmacol. 2001;1:1397–406.

    Article  PubMed  CAS  Google Scholar 

  6. Kim MM, Mendis E, Rajapakse N, Kim SK. Glucosamine sulfate promotes osteoblastic differentiation of MG-63 cells via anti-inflammatory effect. Bioorg Med Chem Lett. 2007;17:1938–42.

    Article  PubMed  CAS  Google Scholar 

  7. Kong CS, Kim JA, Kim SK. Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3–L1 adipocytes. Food Chem Toxicol. 2009;47:2401–6.

    Article  PubMed  CAS  Google Scholar 

  8. Rajapakse N, Mendis E, Kim MM, Kim SK. Sulfated glucosamine inhibits MMP-2 and MMP-9 expressions in human fibrosarcoma cells. Bioorg Med Chem. 2007;15:4891–6.

    Article  PubMed  CAS  Google Scholar 

  9. Mendis E, Kim MM, Rajapakse N, Kim SK. Sulfated glucosamine inhibits oxidation of biomolecules in cells via a mechanism involving intracellular free radical scavenging. Eur J Pharmacol. 2008;579:74–85.

    Article  PubMed  CAS  Google Scholar 

  10. Chun SC, Jee SY, Lee SG, Park SJ, Lee JR, Kim SC. Anti-inflammatory activity of the methanol extract of moutan cortex in LPS-activated Raw264.7 cells. eCAM. 2007;4:327–33.

    PubMed  Google Scholar 

  11. Chen TH, Kao YC, Chen BC, Chen CH, Chan P, Lee HM. Dipyridamole activation of mitogen-activated protein kinase phosphatase-1 mediates inhibition of lipopolysaccharide-induced cyclooxygenase-2 expression in RAW 264.7 cells. Eur J Pharmacol. 2006;541:138–46.

    Article  PubMed  CAS  Google Scholar 

  12. Baeuerle PA, Baichwal VR. NF-kappa B as a frequent target for immunosuppressive and anti-inflammatory molecules. Adv Immunol. 1997;65:111–37.

    Article  PubMed  CAS  Google Scholar 

  13. Asamitsu K, Tetsuka T, Kanazawa S, Okamoto T. RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit. J Biol Chem. 2003;278:26879–87.

    Article  PubMed  CAS  Google Scholar 

  14. Bogdan C. Nitric oxide and the immune response. Nat Immunol. 2001;2:907–16.

    Article  PubMed  CAS  Google Scholar 

  15. Trikha M, Corringham R, Klein B, Rossi JF. Targeted anti-interleukin-6 monoclonal antibody therapy for cancer: a review of the rationale and clinical evidence. Clin Cancer Res. 2003;9:4653–65.

    PubMed  CAS  Google Scholar 

  16. Ii M, Yamamoto H, Adachi Y, Maruyama Y, Shinomura Y. Role of matrix metalloproteinase-7 (matrilysin) in human cancer invasion, apoptosis, growth, and angiogenesis. Exp Biol Med (Maywood). 2006;231:20–7.

    CAS  Google Scholar 

  17. Werz O. 5-Lipoxygenase: cellular biology and molecular pharmacology. Curr Drug Targets Inflam Allergy. 2002;1:23–44.

    Article  CAS  Google Scholar 

  18. Aggarwal BB, Shishodia S, Sandur SK, Pandey MK, Sethi G. Inflammation and cancer: how hot is the link? Biochem Pharmacol. 2006;72:1605–21.

    Article  PubMed  CAS  Google Scholar 

  19. Baldwin AS Jr. The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol. 1996;14:649–83.

    Article  PubMed  CAS  Google Scholar 

  20. Chen TH, Kao YC, Chen BC, Chen CH, Chan P, Lee HM. Withaferin A inhibits iNOS expression and nitric oxide production by Akt inactivation and down-regulating LPS-induced activity of NF-κB in RAW 264.7 cells. Eur J Pharmacol. 2008;599:11–7.

    Article  Google Scholar 

  21. Liu SF, Malik AB. NF-kB activation as a pathological mechanism of septic shock and inflammation. Am J Physiol Lung Cell Mol Physiol. 2006;290:L622–45.

    Article  PubMed  CAS  Google Scholar 

  22. Li YH, Yan ZQ, Jensen JS, Tullus K, Brauner A. Activation of nuclear factor κB and induction of inducible nitric oxide synthase by Ureaplasma urealyticum in macrophages. Infect Immun. 2000;68:7087–93.

    Article  PubMed  CAS  Google Scholar 

  23. Paul A, Cuenda A, Bryant CE, Murray J, Chilvers ER, Cohen P, Gould GW, Plevin R. Involvement of mitogen-activated protein kinasehomologues in the regulation of lipopolysaccharide-mediated induction of cyclo-oxygenase-2 but not nitric oxide synthase in RAW 264.7 macrophages. Cell Signal. 1999;11:491–7.

    Article  PubMed  CAS  Google Scholar 

  24. Pournadeali K. Glucosamine sulfate: a review of efficacy for the pharmacy professional. In J Pharm Comp. 1999;3:428–36.

    Google Scholar 

  25. Van der Kraan PM, de Vries BU, Vitters EL, Van Den Berg WB, Van De Putte LBA. Inhibition of glycosaminoglycan synthesis in anatomically intact rat patellar cartilage by paracetamol-induced serum sulfate depletion. Biochem Pharmacol. 1988;37:368–3690.

    Article  Google Scholar 

  26. Van der Kraan PM, De Vries BU, Vitters EL, De Vries BJ, Van den Berg WB. High susceptibility of human articular cartilage glycosaminoglycan synthesis to changes in inorganic sulfate availability. J Orthop Res. 1990;8:565–71.

    Article  PubMed  Google Scholar 

  27. Zoete V, Bailly F, Maglia F, Rouge′e M, Bensasson RV. Molecular orbital theory applied to the study of non-steroidal anti-inflammatory drug efficiency. Free Radic Biol Med. 1999;26:1261–8.

    Article  PubMed  CAS  Google Scholar 

  28. Arrigoni-Martelli E. Pharmacology of free radical scavenging in inflammation. Int J Tissue React. 1985;7:513–9.

    PubMed  CAS  Google Scholar 

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Acknowledgments

This research was supported by a grant from the Marine Bioprocess Research Center of the Marine Biotechnology Program funded by the Ministry of Land, Transport and Maritime, Republic of Korea.

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Authors and Affiliations

  1. Department of Chemistry, Pukyong National University, Busan, 608-737, Republic of Korea

    Jung-Ae Kim, Byul-Nim Ahn & Se-Kwon Kim

  2. Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan, 617-736, Republic of Korea

    Chang-Suk Kong

  3. Marine Bioprocess Research Center, Pukyong National University, Busan, 608-737, Republic of Korea

    Se-Kwon Kim

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  1. Jung-Ae Kim
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Correspondence to Se-Kwon Kim.

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Responsible Editor: Graham Wallace.

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Kim, JA., Ahn, BN., Kong, CS. et al. Anti-inflammatory action of sulfated glucosamine on cytokine regulation in LPS-activated PMA-differentiated THP-1 macrophages. Inflamm. Res. 60, 1131–1138 (2011). https://doi.org/10.1007/s00011-011-0377-7

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  • DOI: https://doi.org/10.1007/s00011-011-0377-7

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