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Inhibitory effects of lipoteichoic acid from Staphylococcus aureus on platelet function and platelet–monocyte aggregation

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Abstract

Objective

Lipoteichoic acid (LTA) from Staphylococcus aureus has been demonstrated to inhibit agonist-stimulated platelet aggregation. However, its effects on platelet inflammatory mediator release and platelet–monocyte aggregation are still unclear. In the present study, LTA is examined for its anti-inflammatory properties and effects on platelet–monocyte aggregation.

Methods

Blood samples were obtained from 5 healthy volunteers who had taken no medicine in the previous 2 weeks. Washed platelets were prepared and incubated with LTA (0.5–2.0 μg/mL), then platelet aggregation, P-selectin expression, and soluble CD40L (sCD40L) release were measured by light transmission aggregometry, flow cytometry and enzyme-linked immunoassays, respectively. Platelet–monocyte aggregate formation in whole blood was measured by flow cytometry. Thrombin was used as a stimulant.

Results

LTA dose-dependently decreased platelet aggregation from 89.32 ± 10.24% to 36.28 ± 9.01% (P < 0.05), sCD40L release from 3.28 ± 0.76 to 1.13 ± 0.45 ng/mL (P < 0.05) and surface P-selectin expression from 82.01 ± 11.20 to 22.78 ± 6.42% (P < 0.05). In human whole blood, 1.0 μg/mL LTA inhibited platelet–monocyte aggregation from 78.19 ± 10.94 to 38.24 + 8.74% (P < 0.05).

Conclusions

These results indicate that LTA from S. aureus can inhibit platelet-dependent inflammatory mediator release and platelet–monocyte aggregation. These findings suggest that LTA-mediated functional alteration of platelets may contribute to immune evasion of S. aureus.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Number: 30670918) and the Science and Technology Project of Guangdong Province (Number: 0911220600105).

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Authors and Affiliations

  1. Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, Guangzhou, 510630, People’s Republic of China

    Ben-Quan Wu, Ming-Jun Zhi, Hui Liu, Jing Huang, Yu-Qi Zhou & Tian-Tuo Zhang

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  1. Ben-Quan Wu
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  2. Ming-Jun Zhi
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  3. Hui Liu
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Correspondence to Ben-Quan Wu.

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Responsible editor: Makoto Katori.

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Wu, BQ., Zhi, MJ., Liu, H. et al. Inhibitory effects of lipoteichoic acid from Staphylococcus aureus on platelet function and platelet–monocyte aggregation. Inflamm. Res. 60, 775–782 (2011). https://doi.org/10.1007/s00011-011-0333-6

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  • DOI: https://doi.org/10.1007/s00011-011-0333-6

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