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Adjuvant arthritis influences expression of housekeeping genes

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Abstract

Objective and method

We determined whether adjuvant arthritis (AA) affects expression of three housekeeping (HK) genes, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), β-actin and 18s ribosomal RNA (18s rRNA) in organs of Sprague–Dawley rats using the real-time reverse-transcription polymerase chain reaction (RT-PCR).

Results

Expression of 18s rRNA was unaffected by AA whereas that of β-actin was reduced by 52, 62 and 81% in the liver, kidney and intestine, respectively, but not in the heart. AA significantly reduced GAPDH expression in the intestine.

Conclusion

For reliable results, the HK gene must be carefully selected.

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Fig. 1

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Acknowledgments

SH is a recipient of an Egyptian Government scholarship. The study was supported by a grant from the Canadian Institute of Health Research.

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Authors and Affiliations

  1. Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2N8, Canada

    Sherif Hanafy & Fakhreddin Jamali

Authors
  1. Sherif Hanafy
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  2. Fakhreddin Jamali
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Correspondence to Fakhreddin Jamali.

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Responsible Editor: John Di Battista.

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Hanafy, S., Jamali, F. Adjuvant arthritis influences expression of housekeeping genes. Inflamm. Res. 60, 521–523 (2011). https://doi.org/10.1007/s00011-011-0327-4

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  • DOI: https://doi.org/10.1007/s00011-011-0327-4

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