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Quercetin and kaempferol suppress immunoglobulin E-mediated allergic inflammation in RBL-2H3 and Caco-2 cells

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Abstract

Objective

We investigated the inhibitory effects of quercetin and kaempferol treatment on the suppression of immunoglobulin E (IgE)-mediated allergic responses in relation to intestinal epithelium barrier function in RBL-2H3 and Caco-2 cells.

Methods

RBL-2H3 cells as a model of intestinal mucosa mast cells were treated with flavonols followed by IgE-anti-dinitrophenyl sensitization. The extent of degranulation and the release of pro-inflammatory cytokines were measured. Caco-2 cells were stimulated with interleukin (IL)-4 or IgE-allergen with or without flavonol pretreatment and changes in the expression of CD23 mRNA and mitogen-activated protein kinase (MAPK), and chemokine release were determined.

Results

Flavonols inhibited the secretion of allergic mediators in RBL-2H3 cells and suppressed the CD23 mRNA expression and p38 MAPK activation in IL-4 stimulated Caco-2 cells. Flavonols also suppressed IgE-OVA induced extra signal-regulated protein kinase (ERK) activation and chemokine release.

Conclusions

Quercetin and kaempferol effectively suppressed the development of IgE-mediated allergic inflammation of intestinal cell models.

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Acknowledgments

This study was supported by the grant (A080664) from the Ministry for Health, Welfare and Family Affairs, Republic of Korea.

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Authors and Affiliations

  1. Department of Food and Nutrition, Sookmyung Women’s University, Seoul, 140-742, Korea

    Eun-Ju Lee & Mi-Kyung Sung

  2. Department of Food and Nutrition, Seoul National University, Seoul, Republic of Korea

    Geun-Eok Ji

  3. Research Center, BIFIDO Co., Ltd, Seoul, Republic of Korea

    Geun-Eok Ji

Authors
  1. Eun-Ju Lee
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Correspondence to Mi-Kyung Sung.

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Responsible Editor: A. Falus.

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Lee, EJ., Ji, GE. & Sung, MK. Quercetin and kaempferol suppress immunoglobulin E-mediated allergic inflammation in RBL-2H3 and Caco-2 cells. Inflamm. Res. 59, 847–854 (2010). https://doi.org/10.1007/s00011-010-0196-2

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  • DOI: https://doi.org/10.1007/s00011-010-0196-2

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