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Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats

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Abstract

Objective

We report the effects of the gastrin-releasing peptide (GRP) receptor antagonist RC-3095 in an acute inflammation model induced by carrageenan.

Methods

Male Wistar rats received saline or saline containing 2% λ-carrageenan into the pleural cavity, with some also receiving RC-3095 3 mg/kg subcutaneously, immediately after surgery. Four hours later, the rats were killed and pleural exudate was obtained for evaluation of total cell count, lactate dehydrogenase activity, total protein, cytokines analysis and nitrite/nitrate concentrations; myeloperoxidase (MPO) activity and oxidative stress were evaluated in the lung.

Results

RC-3095 exhibited pronounced anti-inflammatory actions by inhibition of leukocyte influx and blockade of MPO, nitrite/nitrate and cytokine levels. Moreover, the results showed that RC-3095 elicits action against oxidative damage in lipids and proteins, as well as increasing cell viability.

Conclusion

The present findings suggest that GRP plays a role in acute inflammation that can be related with the reduction of oxidative damage and that it could be effective in therapeutic applications.

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Acknowledgments

This work was supported by grants from Universidade do Extremo Sul Catarinense (UNESC), Coordenação de Aperfeiçoamento de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Experimental Physiopathology Laboratory, Postgraduate Program in Health Sciences, University of the Extreme-South Catarinense, 88006-000, Criciúma, SC, Brazil

    Fabricia Petronilho, Bruna de Souza, Francieli Vuolo, César A. F. Benetton, Emilio L. Streck & Felipe Dal-Pizzol

  2. Department of Biochemistry, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, 90046-900, Porto Alegre, RS, Brazil

    Fabricia Petronilho & Felipe Dal-Pizzol

  3. Cellular and Molecular Neuropharmacology Research Group, Department of Pharmacology Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, 90046-900, Porto Alegre, RS, Brazil

    Rafael Roesler

  4. Department of Internal Medicine, Faculty of Medicine, Federal University of Rio Grande do Sul, 90035-003, Porto Alegre, RS, Brazil

    Gilberto Schwartsmann

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  1. Fabricia Petronilho
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  2. Bruna de Souza
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  5. Emilio L. Streck
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  7. Gilberto Schwartsmann
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  8. Felipe Dal-Pizzol
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Corresponding author

Correspondence to Felipe Dal-Pizzol.

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Responsible Editor: I. Ahnfelt-Rønne.

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Petronilho, F., de Souza, B., Vuolo, F. et al. Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats. Inflamm. Res. 59, 783–789 (2010). https://doi.org/10.1007/s00011-010-0190-8

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  • DOI: https://doi.org/10.1007/s00011-010-0190-8

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