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Protective effects of emodin combined with danshensu on experimental severe acute pancreatitis

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Abstract

Objective and design

In the present experiment, we aimed to determine the feasibility and curative effects of emodin combined with danshensu on experimental severe acute pancreatitis (SAP) and the mutual benefit of this synergistic strategy by a prospective animal study.

Material

Eighty Wistar rats were randomly divided into four groups (n = 20).

Treatment

SAP was elicited by a retrograde infusion of 5.0% sodium taurocholate into the pancreatic main duct. SAP rats in each group received no further intervention, emodin alone, danshensu (DSS) alone, and emodin combined with DSS (EDSS), respectively.

Methods

48 h after SAP induction, all surviving animals were sacrificed to collect blood and tissue samples for the following measurements: serum levels of amylase, tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), endotoxin and D-lactate. Pancreatic levels of TNF-α, IL-1β, maleic dialdehyde (MDA), myeloperoxidase (MPO) activity, nuclear factor-κappaB (NF-κB) activation as well as wet-dry weight ratio were also evaluated. Ascitic fluid was quantified and the severity of pancreatic damage was analyzed by pathological grading and scoring.

Results

Compared with the SAP group, the emodin, DSS and EDSS groups had significant differences in every index. Furthermore, EDSS obviously improved all the parameters mentioned above so as to counteract inflammatory response and oxidative stress, as well as most effectively abating pancreatic and intestinal barrier injury.

Conclusions

EDSS exerted protective effects on SAP rats and remarkably alleviated the severity of experimental SAP. Mechanisms that might account for the beneficial effects include protecting the intestinal barrier, inhibiting over-inflammatory reaction and abating oxidative stress. The combined strategy proved to be more effective than either emodin or DSS alone and may cause synergistic effects in combination in the early stage of SAP. Broad potential for future clinical practice is foreseeable.

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Acknowledgments

The authors would like to thank Ms.LIU Mei-na for her help with statistical analysis. This work was supported in part by grants from New Century Support Foundation for Elitist of Chinese Ministry of Education (NCET-07-0248), Scientific Foundation for Prominent Youth of Heilongjiang Province, China (JC200717), Scientific and Technical Foundation from Department of Education of Heilongjiang Province, China (11521127) and Scientific Foundation of the First Affiliated Hospital of Harbin Medical University, China (2007006).

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Authors and Affiliations

  1. Department of Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng St, Nangang Dist, 150001, Harbin, Heilongjiang, People’s Republic of China

    Gang Wang, Bei Sun, Dongbo Xue & Hongchi Jiang

  2. Department of Pathology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng St, Nangang Dist, 150001, Harbin, Heilongjiang, People’s Republic of China

    Hong Zhu

  3. Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden

    Yue Gao

  4. Department of Pathology, Hospital of Heilongjiang Province, 82 Zhongshan St, Xiangfang Dist, 150036, Harbin, Heilongjiang, People’s Republic of China

    Xiurong Li

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  1. Gang Wang
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Correspondence to Bei Sun.

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Responsible Editor: A. Bauhofer

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Wang, G., Sun, B., Zhu, H. et al. Protective effects of emodin combined with danshensu on experimental severe acute pancreatitis. Inflamm. Res. 59, 479–488 (2010). https://doi.org/10.1007/s00011-009-0152-1

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  • DOI: https://doi.org/10.1007/s00011-009-0152-1

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