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Plasma calprotectin in chronically dialyzed end-stage renal disease patients

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Abstract

Objective

The current study aimed to evaluate plasma calprotectin levels and clearance end-stage renal disease (ESRD) patients with and without acute infection undergoing chronic hemodialysis (HD).

Materials and methods

Blood samples from 54 HD patients were obtained before and after the HD and 42 healthy blood donors were examined as controls. The blood levels of calprotectin, procalcitonin, C-reactive protein (CRP), and intracellular production of interleukins 10 and 12 in monocytes were determined in both groups.

Results

The concentrations of plasma calprotectin in ESRD patients were significantly higher than in healthy controls (p < 0.05). No differences between pre- and post-HD calprotectin plasma levels were observed (p = 0.07 for two-tailed test). Plasma calprotectin levels were not significantly influenced by the presence of acute infection (p = 0.19) or diabetes (p = 0.42). A significant positive correlation of plasma calprotectin to plasma beta-2 microglobulin was proven (p < 0.05). Procalcitonin (PCT), CRP, IL-10, and IL-12 were not correlated with plasma calprotectin before or after HD. The elevation of plasma calprotectin was correlated strongly to the hemodialysis vintage (r = 0.55, p < 0.01).

Conclusions

Significantly elevated levels of plasma calprotectin in ESRD patients occur without an acute infectious cause and are not affected by the presence of diabetes. By analogy to plasma beta-2 microglobulin, a close relation of plasma calprotectin to HD vintage was shown.

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Acknowledgments

This project was supported by Research Project No. 0021620807 of the Czech Ministry of Education.

Conflict of interest statement

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Institute of Clinical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic

    Karin Malíčková, Helena Brodská, Sylvie Dusilová Sulková, Ivana Janatková, Helena Marečková & Tomáš Zima

  2. Department of Nephrology, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic

    Jana Lachmanová & Vladimír Tesař

  3. Department of Gerontology and Metabolism, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic

    Sylvie Dusilová Sulková

  4. Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic

    Helena Marečková

  5. Institute of Clinical Biochemistry and Laboratory Diagnostics, Laboratory of Clinical Immunology and Allergology, First Faculty of Medicine, Charles University and General University Hospital, Karlovo nam. 32, 12111, Prague 2, Czech Republic

    Karin Malíčková

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  1. Karin Malíčková
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  2. Helena Brodská
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Correspondence to Karin Malíčková.

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Responsible Editor: C. Kasserra.

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Malíčková, K., Brodská, H., Lachmanová, J. et al. Plasma calprotectin in chronically dialyzed end-stage renal disease patients. Inflamm. Res. 59, 299–305 (2010). https://doi.org/10.1007/s00011-009-0103-x

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  • DOI: https://doi.org/10.1007/s00011-009-0103-x

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