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feG-COOH blunts eosinophilic airway inflammation in a feline model of allergic asthma

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Abstract

Objective and design

This study investigated if feG-COOH would decrease allergen-induced airway inflammation.

Materials or subjects

Seven adult cats sensitised to Bermuda grass allergen (BGA) to induce an asthmatic phenotype.

Treatment

Cats were randomized to receive either feG-COOH (1 mg/kg, PO) or placebo (saline 1 ml, PO) immediately prior to BGA aerosol challenge in a cross-over design.

Methods

Bronchoalveolar lavage fluid (BALF) was collected and airway inflammatory response assessed via inflammatory cell number and type; IL-4, IFN-γ and nitric oxide metabolite concentrations. A paired t test was used to compare parameters with a P < 0.05 considered significant.

Results

The BALF eosinophil percentage was significantly lower in asthmatic cats treated with feG compared with placebo (placebo, 35.3 ± 12.2%; feG, 22.4 ± 8.6%; P = 0.002). Treatment with feG did not result in a significant change in any other parameter measured.

Conclusions

These data indicate that a single dose of feG-COOH partially attenuates eosinophilic airway inflammation in experimental feline asthma.

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Acknowledgments

The authors would like to thank Craig Woods and Imulan BioTherapeutics for their generous donation of feG-COOH. Additionally, the authors appreciate the assistance of Rachael Cohen this project.

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Author notes

  1. Elizabeth Schooley

    Present address: Veterinary Referral and Critical Care, 1596 Hockett Rd., Manakin-Sabot, VA, 23103, USA

Authors and Affiliations

  1. Department of Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 E. Campus Drive, Columbia, MO, 65211, USA

    Amy E. DeClue, Elizabeth Schooley, Laura A. Nafe & Carol R. Reinero

Authors
  1. Amy E. DeClue
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  2. Elizabeth Schooley
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  3. Laura A. Nafe
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  4. Carol R. Reinero
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Corresponding author

Correspondence to Carol R. Reinero.

Additional information

Responsible Editor: M. Parnham.

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DeClue, A.E., Schooley, E., Nafe, L.A. et al. feG-COOH blunts eosinophilic airway inflammation in a feline model of allergic asthma. Inflamm. Res. 58, 457–462 (2009). https://doi.org/10.1007/s00011-009-0010-1

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  • DOI: https://doi.org/10.1007/s00011-009-0010-1

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