Hydrogen sulphide is pro-inflammatory in haemorrhagic shock

Abstract:

Objective:

H₂S is pro-inflammatory in inflammatory models, thus we investigated whether H₂S plays a role in haemorrhagic shock (HS)-associated inflammation.

Methods:

Male, Sprague-Dawley rats were given an inhibitor of H₂S biosynthesis, DL-propargylglycine (PAG, 50 mg/kg, i. v.) or saline (1 ml/kg) 30 min before blood withdrawal and subjected to HS (mean arterial pressure (MAP) of 40 mM Hg for 90 min) followed by reinfusion of shed blood. Animals were killed at 5, 90, 270 and 630 min after reinfusion.

Results:

Pre-treatment of animals with PAG 1) increased the HR recovery rate (n = 6 – 12, P < 0.05); 2) attenuated the increase in plasma levels of TNF-α and IL-6 and reduced lung iNOS expression levels (n =5 P, < 0.05); and 3) attenuated the increase in plasma levels of ALT and reduced HS-induced increase in liver and lung myeloperoxidase (MPO) activity (n = 5, P < 0.05).

Conclusions:

H₂S is pro-inflammatory in HS and inhibition of H₂S biosynthesis may reduce some HS-induced inflammatory responses and organ injury.