Abstract.
Objective:
To investigate endotoxin-induced tolerance, intracellular cytokine synthesis polarization and monocyte apoptosis during Familial Mediterranean Fever (FMF).
Methods:
Lipopolysaccharide (LPS)-induced tolerance, intracellular cytokine synthesis and monocyte apoptosis were determined in FMF patients by flow cytometry using whole blood cell culture technique.
Results:
Endotoxin homo- and cross-tolerance, detected as the percentage of TNF-α synthesizing monocytes, developed in whole blood preparations of patients in attack period, but not during remission. The induction of anti-inflammatory cytokine synthesis polarization and enhancement of iodine-lithium-α-dextrin- and LPS-induced monocyte apoptosis was observed in FMF patients during the attack, whereas monocytes from patients in remission period exhibited proinflammatory cytokine polarization and resistance to the repeated LPS-induced apoptosis. Colchicine induced anti-inflammatory cytokine synthesis and caused down-modulation of monocyte apoptosis, whereas cytokines did not alter LPS-induced monocyte apoptosis.
Conclusion:
The self-limited nature of attacks during FMF may represent periods of inflammation resolution compensatory to continued sub-clinical inflammation during the remission.
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Received 12 April 2007; returned for revision 25 June 2007; accepted by C. Kasserra 26 August 2007
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Davtyan, T.K., Hakobyan, G.S., Avetisyan, S.A. et al. Engaging anti-inflammatory mechanisms and triggering inflammatory effector apoptosis during Familial Mediterranean Fever attack. Inflamm. res. 57, 65–74 (2008). https://doi.org/10.1007/s00011-007-7074-6
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DOI: https://doi.org/10.1007/s00011-007-7074-6