Skip to main content
Log in

Induction of a novel boiling stable protein in response to desiccation and ABA treatments in Sesbania sesban var. bicolor leaves

  • Published:
Biologia Plantarum

Abstract

Several polypeptides were induced in leaves of Sesbania sesban var. bicolor under water stress (desiccation). Among them, the SDS-PAGE resolved a few high molecular mass polypeptides along with one major of 66 kDa. After boiling the total protein fraction, some low molecular mass polypeptides (10 – 30 kDa) as well as the one of 66 kDa remained stable. The latter (66 kDa) polypeptide is also regulated by exogenous application of ABA, indicating its significant role in adaptation of sesban to drought.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Abbreviations

ABA:

abscisic acid

EDTA:

ethylenediaminetetraacetic acid

FM:

fresh mass

References

  • Baker, J.C., Steele, C., Dure, L.: Sequence and characterization of 6 LEA proteins and their genes from cotton.-Plant mol. Biol. 11: 277–291, 1988.

    Article  CAS  Google Scholar 

  • Barathi, P., Sundar, D., Ramachandra Reddy, A.: Changes in mulberry leaf metabolism in response to water stress.-Biol. Plant. 44: 83–87, 2001.

    Article  CAS  Google Scholar 

  • Bewley, J.D., Larsen, K.M.: Cessation of protein synthesis in water-stressed pea roots and maize mesocotyls without loss of polyribosomes. Effects of lethal and non-lethal water stress.-J. exp. Bot. 31: 1245–1256, 1980.

    CAS  Google Scholar 

  • Close, T.J., Chandler, P.M.: Cereal dehydrins: serology, gene mapping and potential functional roles.-Aust. J. Plant Physiol. 17: 333–344, 1990.

    Article  CAS  Google Scholar 

  • Close, T.J., Kortt, A.A., Chandler, P.M.: A cDNA based comparison of dehydration-induced proteins (dehydrins) in barley and corn.-Plant mol. Biol. 13: 95–108, 1989.

    Article  CAS  PubMed  Google Scholar 

  • Ingram, J., Bartels, D.: The molecular basis of dehydration tolerance in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 377–403, 1996.

    Article  CAS  PubMed  Google Scholar 

  • Jacobsen, J.V., Shaw, D.C.: Heat-stable proteins and abscisic acid action in barley aleurone cells.-Plant Physiol. 91: 1520–1526, 1989.

    Article  CAS  PubMed  Google Scholar 

  • Laemmli, U.K.: Cleavage of structural protein during the assembly of the head of bacteriophage 4.-Nature 227: 680–685, 1970.

    Article  PubMed  CAS  Google Scholar 

  • McCue, K.F., Hanson, A.D.: Drought and salt tolerance: towards understanding and application.-Trends Biotechnol. 8: 358–362, 1990.

    Article  CAS  Google Scholar 

  • Mundy, J., Chua, N.H.: Abscisic acid and water stress induce the expression of a novel rice gene.-EMBO J. 7: 2279–2286, 1988.

    CAS  PubMed  Google Scholar 

  • Pelah, D., Shoseyov, O., Altman, A.: Characterization of BspA, a major boiling-stable, water-stress-responsive protein in aspen (Populus tremula).-Tree Physiol. 15: 673–678, 1995.

    CAS  PubMed  Google Scholar 

  • Peterson, G.L.: A simplification of the protein assay method of Lowry et al. which is more generally applicable.-Anal. Biochem. 83: 346–356, 1979.

    Article  Google Scholar 

  • Pinedo, M.L., Hernández, G.F., Conde, R.D., Tognetti, J.A.: Effect of low temperature on the protein metabolism of wheat leaves.-Biol. Plant. 43: 363–367, 2000.

    Article  CAS  Google Scholar 

  • Reviron, M.P., Vartanian, N., Sallantin, M., Huet, J.C., Pernollet, J.C., de Vienne, D.: Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves.-Plant Physiol. 100: 1486–1493, 1992.

    Article  CAS  PubMed  Google Scholar 

  • Roy-Macauley, H., Zuily-Fodil, Y., Kidric, M., Pham Thi, A.T., Vieira da Silva, J.: Effect of drought stress on proteolytic activities in Phaseolus and Vigna leaves from sensitive and resistant plants.-Physiol. Plant. 85: 90–96, 1992.

    Article  CAS  Google Scholar 

  • Skriver, K., Mundy, J.: Gene expression in response to abscisic acid and osmotic stress.-Plant Cell 2: 503–512, 1990.

    Article  CAS  PubMed  Google Scholar 

  • Sundar, D., Chaitanya, K.V., Ramachandra Reddy, A.: Stress-induced proteins in Parthenium argentatum leaves.-Biol. Plant. 46: 313–316, 2003.

    Article  CAS  Google Scholar 

  • Valluri, J.V., Treat, W.J., Newton, R.J., Cobb, B.G., Soltes, E.J.: Protein synthesis in slash pine callus cultures exposed to water stress.-Tree Physiol. 4: 181–186, 1998.

    Google Scholar 

  • Vilardell, J., Goday, A., Freire, M.A., Torrent, M., Martinez, C., Torne, J.M., Pages, M.: Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize.-Plant mol. Biol. 14: 423–432, 1990.

    Article  CAS  PubMed  Google Scholar 

  • Vu, C.V., Allen, L.H., Garrard, L.A.: Effects of supplemental UV-B radiation on primary photosynthesis carboxylating enzymes and soluble proteins in leaves of C3 and C4 crop plants.-Physiol. Plant. 55: 11–16, 1982.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. C. Gupta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Purty, R.S., Agrawal, V. & Gupta, S.C. Induction of a novel boiling stable protein in response to desiccation and ABA treatments in Sesbania sesban var. bicolor leaves. Biol Plant 49, 137–140 (2005). https://doi.org/10.1007/s00000-005-7140-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00000-005-7140-1

Additional key words

Navigation