Abstract
Somatic embryogenesis was initiated from immature zygotic embryos of intraspecific cross ofAbies alba and interspecific combination ofAbies alba x Abies nordmanniana. The most responsive explants for induction of embryogenic calli were zygotic embryos in precotyledonary stage of development. Biochemically the compared embryogenic lines were uniform irrespective of their morphology or embryogenic potential. The flow cytometric analysis of single embryogenic lines suggests that no changes in ploidy levels occur during induction and culture of embryogenic callus inAbies alba what confirms the convenience of this system for propagation of valuable seed material.
Similar content being viewed by others
References
Doležel, J.: FLOWSTAR: A microcomputer program for flow cytometric data manipulation and analysis.—Biológia (Bratislava)44: 287–291, 1989.
Doležel, J.: Flow cytometric analysis of nuclear DNA content in higher plants.—Phytochem. Anal.2: 143–154, 1991.
Doležel, J., Binarová, P., Lucretti, S.: Analysis of nuclear DNA content in plant cells by flow cytometry.—Biol. Plant.31: 113–120, 1989.
Fransz, P.F.: Isozymes as biochemical and cytochemical markers in embryogenic callus cultures ofZea mays.—Med. Landbouwuniversiteit (Wageningen)20: 91–100, 1988.
Gebhardt, K., Weisgerber, H., Fröhlich, H.J.:In vitro germination and production of embryogenic callus from liquid suspension cultures ofAbies alba.—In: Proc. Int. Conifer Tissue Cult. Working Group, August 8–12. Saskatoon 1988.
Hakman, I., Von Arnold, S., Fellner-Feldegg, H.: Isolation and DNA analysis of protoplasts from developing female gametophytes ofPicea abies (Norway spruce).—Can. J. Bot.64: 108–112, 1986.
Hristoforoglu, K., Grahsl, A., Schmidt, J.: Somatic embryogenesis from mature embryos ofAbies alba.—In: Int. Conifer Biotech. Working Group at Radisson Governors Inn, April 23–28. Research Triangle Park, Saskatoon 1992.
Joersbo, M., Andersen, J.M., Okkels, F.T., Rajagopal, R.: Isoperoxidases as markers of somatic embryogenesis in carrot cell suspension cultures.—Physiol. Plant.76: 10–16, 1989.
Klimaszewska, K.: Plantlet development from immature zygotic embryos of hybrid larch through somatic embryogenesis.—Plant Sci.63: 95–103, 1989.
Kochba, J., Lavee, S., Spiegel-Roy, P.: Differences in peroxidase activity and isozymes in embryogenic and non-embryogenic “shamonti” organe ovular callus lines.—Plant Cell Physiol.18: 463–467, 1977.
Kormuták, A.: Possibilities of extending the silver fir (A. alba Mill.) genepool by means of interspecific hybridization.—In: Kormuták, A. Užák, D. (ed.): Proceedings of Symp. Genepool of Forest Woody Species, its Conservation and Utilization. Pp. 341–353, Nitra 1986.
Libiaková, G., Dediĉová, B.: Regeneration of protoplasts derived from callus cultures ofAbies sp. Biológia (Bratislava)50: in press, 1995.
Libiaková, G., Gajdoŝová, A., Vooková, B., Kubincová, M., Doležel, J.: Karyological study ofAbies concolor × Abies grandis calli and shoots regeneratedin vitro.—Biológia (Bratislava)50: in press, 1995.
Medgyesy, P., Menzel, L., Maliga, P.: The use of cytoplasmic streptomycin resistance: chloroplast transfer fromNicotiana tabacum intoNicotiana sylvestris and isolation of their somatic hybrids.— Mol. gen. Genet.179: 693–698, 1980.
Mo, L.H., Egeilsdollet, U., Von Arnold, S.: Differences in morphology and extracellular proteins among embryogenic cell-lines ofPicea abies.—In: Int. Conifer Biotech. Working Group at Radisson Governors Inn, April 23–28. Research Triangle Park, Saskatoon 1992.
Mo, L.H., Von Arnold, S., Lagercrantz, U.: Morphogenic and genetic stability in longterm embryogenic cultures and somatic embryos of Norway spruce (Picea abies (L.) Karst).—Plant Cell Rep.8: 375–378, 1989.
Noh, E.W., Minocha, S.C.: Pigment and isozyme variation in aspen shoots regenerated from callus culture.—Plant Cell Tissue Organ Cult.23: 39–44, 1990.
Norgaard, J.V., Krogstrup, P.: Cytokinin induced somatic embryogenesis from immature embryos ofAbies nordmanniana Lk..—Plant Cell Rep.9: 509–513, 1991.
Rao, K.V., Suprasanna, P., Reddy, G.M.: Biochemical changes in embryogenic and non-embryogenic calli ofZea mays L..—Plant Sci.66: 127–130, 1990.
Salajová, T., Salaj, J.: Somatic embryogenesis in European black pine (Pinus nigra Arn.).—Biol. Plant.34: 213–218, 1992.
Schuller, A., Reuther, G., Geier, T.: Somatic embryogenesis from seed explants ofAbies alba.— Plant Cell Tissue and Organ Cult.17: 53–58, 1989.
Wann, S.R., Johnson, M.A., Noland, T.L., Carlson, J.A.: Biochemical differences between embryogenic and non-embryogenic callus ofPicea abies (L.) Karst..—Plant Cell Rep.6: 39–42, 1987.
Wyman, J., Brassard, N., Flipo, D., Laliberté, S.: Ploidy level stability of callus tissue, axillary and adventitious shoots ofLarix x eurolepis Henry regeneratedin vitro.—Plant Sci.85: 189–196, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gajdošová, A., Vooková, B., Kormuťák, A. et al. Induction, protein composition and DNA ploidy level of embryogenic calli of silver fir and its hybrids. Biol Plant 37, 169–176 (1995). https://doi.org/10.1007/BF02913205
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02913205