Summary
Three new continuous cell lines were established from two species of marine fishes economically important in Asia. Perch (Lateolabrax japonicus) heart (PH), perch liver (PL), and grouper (Epinephelus amblycephalus) kidney (GK) lines were established in Eagle’s minimum essential medium with 10% fetal bovine serum and have been subcultured over 120 times. The optimum growth temperature was 25°C for the PK and GK lines and 30° C for the PH line. The modal chromosome numbers for each cell line are: PH (49), PK (50), and GK (65). None of the lines was susceptible to the rhabdovirus infectious hematopoietic necrosis virus (IHNV) or channel catfish herpesvirus (CCV); however, all three cell lines were susceptible to a variety of fish birnaviruses, including infectious pancreatic necrosis virus (IPNV), the EVE eel virus, and newly isolated birnaviruses from a variety of fish and shellfish in Taiwan.
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Bowser, P. R.; Plumb, J. A. Growth rates of a new cell line from channel catfish ovary and channel catfish virus replication at different temperatures, Can. J. Fish. Aqua. Sci. 37:871–873; 1980.
Chen, T. R. Microscopic demonstration of mycoplasma contamination in cell culture and cell culture media. TCA Manual 1:229–232; 1975.
Clem, L. W.; Moewus, L.; Sigel, M. M. Studies with cells from marine fish in tissue culture. Proc. Soc. Exp. Biol. Med. 108:762–766; 1961.
Karber, G. Beitrag zur Kollektiven behandburg pharmakologischer reihenverswche. Arch. Ex. Pathol. Pharmakol. 162:480–483; 1931.
Nicholson, B. L. Techniques in fish cell culture. In: Kurstak, E., ed. Techniques in cell biology. Amsterdam: Elsevier, V. Cl. C105:1–16; 1985.
Watanabe, Y.; Hanada, H., Ushiyama, M. Monolayer culture from marine fish. Fish Pathol. 15:201–205; 1981.
Wolf, K.; Mason, J. A. Poikilothermic vertebrate cell lines and viruses. A current listing for species. In Vitro 16:168–179; 1980.
Wolf, K.; Quimby, M. C. Established eurythermic line of fish cells in vitro. Science 135:1065–1066; 1962.
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This research was funded by National Science Foundation grant INT-810447, the University of Main/University of New Hampshire Sea Grant College Program, and the Maine Agriculture Experiment Station publication no. 1165.
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Nicholson, B.L., Danner, D.J. & Wu, J.L. Three new continuous cell lines from marine fishes of asia. In Vitro Cell Dev Biol 23, 199–204 (1987). https://doi.org/10.1007/BF02623580
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DOI: https://doi.org/10.1007/BF02623580