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Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection

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Summary

A novel method for the simultaneous determination of twelve water- and fat-soluble vitamins has been established by high-performance liquid chromatography with diode array detection. The vitamins were analyzed on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol-KH2PO4 buffer (0.1 M, pH 7.0)-water as mobile phase in a gradient. The linearity of calibration graphs was compound-dependent and the detection limits ranged from 0.02 μg mL−1 to 0.5 μg mL−1. The method was successfully applied to determine vitamins in pharmaceutical preparations. The recoveries were from 95.1% to 103% and the relative standard deviations were in the range of 0.9% to 4.5%.

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Authors and Affiliations

  1. Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong

    H. B. Li & F. Chen

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  1. H. B. Li
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  2. F. Chen
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Li, H.B., Chen, F. Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection. Chromatographia 54, 270–273 (2001). https://doi.org/10.1007/BF02492256

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  • DOI: https://doi.org/10.1007/BF02492256

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