Summary
A new method was developed for the determination of fifteen biogenic amines in the intestinal digesta and feces of animal or human origin. The method involves the addition of an internal standard (heptylamine), extraction of the amines, and precipitation of the proteins with perchloric acid. The amines are derivatised with dansyl chloride, separated on a C18 column using gradient elution with 0.2M ammonium acetate at pH 5, water and acetonitrile, and detected with a fluorescence detector. The separation was achieved in a 40 min run. Recoveries ranged from 67 to 110%, the relative standard deviation for intra-assay precision being <5% and the limit of determination 1–5 mg kg−1. The method is specific for biogenic amines in intestinal and fecal samples.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Smith, E.A.; Macfarlane, G.T.Anaerobe 1996,2, 285.
Macfarlane, G.T.; Cummings, J.H., inThe large intestine: Physiology, Patholphysiology and Disease edited by Philips, S.F.; Pemberton, J.H.; Shorter, R.G., Raven Press., Ltd., New York1991, 81.
Schneider, R.; Kreienbring, F.; Bolduan, G.; Beck, M.Arch. Anim. Nutr. 1989,39, 1021.
Phuntsok, T.; Froetschel, M.A.; Amos, H.E.; Zheng, M.; Huang, Y.W.,J. Dairy Sci. 1998,81, 2193.
Bermudez, A.J.; Firman, J.D.Avian diseases 1998,42, 199.
Desiderio, M.A.; Davalli, P.; Perin, A.J. Chromatogr. 1987,419, 285.
Kabra, P.M.; Lee, H.K.J. Chromatogr. 1986,380, 19.
Lehtonen, P.Z. Lebensm. Unters. Forsch. 1986,183, 177.
Rosier, J.; Van Peteghem, C.Z. Lebensm. Unters. Forsch. 1988,186, 25.
Gennaro, M.C.; Mentasti, E.; Sarzanini, C.; Porta, V.Chromatographia 1988,25, 117.
Moret, S.; Bortolomeazzi, R. Improved extraction procedure for biogenic amines in foods and their high-performance liquid chromatographic determinationJ. Chromatogr. 1992,591, 175.
Eerola, S.; Hinkkanen, R.; Lindfors, E.; Hirvi, T.J. AOAC Int. 1993,76 575.
Chiavari, G.; Galletti, G.C.; Vitali, P.Chromatographia 1989,27, 216.
Bermudez, A.J.; Firman, J.D.Avian diseases 1998,42, 199.
Eckel, V.B.; Roth, F.X.; Kirchgessner, M.; Eidelsburger, U.J. Anim. Physiol. a. Anim. Nutr. 1992,67, 198.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saarinen, M.T. Determination of biogenic amines as dansyl derivatives in intestinal digesta and feces by reversed phase HPLC. Chromatographia 55, 297–300 (2002). https://doi.org/10.1007/BF02491662
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02491662