Summary
Capillary electrochromatography is an emerging technique utilising small diameter capillaries (typically 50 μm) packed with HPLC-type stationary phases. Using electrically driven flow, higher efficiencies can be obtained than with pumped flow. This paper discusses some important points to enable more efficient, reliable capillaries to be prepared and also offers some suggestions regarding more suitable choices of mobile phase buffer which suffer less “depletion” problems than those buffers used to date. Separations of some drug candidates are shown and the future development of this technique is briefly discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J. H. Knox, I. H. Grant, Chromatographia24, 135 (1987).
J. H. Knox Chromatographia26, 329 (1988).
J. H. Knox, I. H. Grant, Chromatographia32, 317 (1991).
H. Yamamoto, J. Baumann, F. Erni, J. Chromatogr.593, 313 (1992).
N. W. Smith, M. B. Evans, Chromatographia38, 649 (1994).
H. Rebscher, U. Pyell, Chromatographia38, 737 (1994).
R. T. Kennedy, J. W. Jorgenson, Anal. Chem.61, 1128 (1989).
S. Hoffmann, L. Blomberg, Chromatographia24, 416 (1987).
A. Shafaati, B. J. Clark, Anal. Proceedings30, 481 (1993).
Tao Zhu, Yi-liang Sun, Choa-xuan Zhang, Da-kui Ling, Zeng-pei Sun, J. High Res. Chromatogr.17, 563 (1994).
A. Malik, W. Li, M. Lee, J. Microcol. Sep.5, 361 (1993).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Boughtflower, R.J., Underwood, T. & Paterson, C.J. Capillary electrochromatography — Some important considerations in the preparation of packed capillaries and the choice of mobile phase buffers. Chromatographia 40, 329–335 (1995). https://doi.org/10.1007/BF02290365
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02290365