Summary
Regeneration of plants from protoplasts is regarded a difficult and lengthy procedure which requires well developed skills on the side of the experimenter. Therefore, where alternative procedures for genetic engineering of plants are available, protoplast-based techniques are frequently avoided. Here, we demonstrate, that by our newly developed thin-alginate-layer technique it is possible to regenerate shoots from leaf protoplasts ofNicotiana tabacum L. at very high efficiency and very rapidly, with the first shoots appearing within less than two weeks. Root formation is induced on a third medium with first roots being found after only 10 more days of culture.
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Dovzhenko, A., Bergen, U. & Koop, H.U. Thin-alginate-layer technique for protoplast culture of tobacco leaf protoplasts: Shoot formation in less than two weeks. Protoplasma 204, 114–118 (1998). https://doi.org/10.1007/BF01282299
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DOI: https://doi.org/10.1007/BF01282299