Abstract
Electrophysiological recordings from antennal olfactory receptor cells were obtained fromIps grandicollis. Recordings were made from olfactory receptor cells from nine regions of the antennae in response to stimulation with the semiochemicalsα-pinene, frontalin,endo-brevicomin, verbenone,trans-verbenol,cis-verbenol, ipsdienol, and ipsenol. In many cases, up to two cells were recorded concurrently from the same location. When compared to males, females had a greater percentage of cells responsive to the primary pheromones ofDendroctonus frontalis, frontalin andtrans-verbenol, and ofIps spp., ipsdienol and ipsenol. Among females, more cells responded totrans-verbenol and theIps-produced volatiles than to host or otherD. frontalis-produced compounds. Olfactory cells of males responded mostly tocis-verbenol, followed byα-pinene, verbenone,trans-verbenol, andendo-brevicomin. Of those cells responsive primarily to one compound, the greatest percentage were responsive totrans-verbenol in females and to verbenone in males. The response of the antennal olfactory receptor cells to semiochemicals used by male and femaleI. grandicollis is consistent with the presence of these compounds during the host colonization period for each sex. Our results, which show a lack of specificity in most pheromone and host odor receptor cells, is in contrast with previously published accounts of olfactory receptor cell specificity in otherIps species.
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References
Billings, R.F. 1985. Southern pine bark beetles and associated insects: Effects of rapidly released host volatiles on response to aggregation pheromones.Z. Angew. Entomol. 99:483–491.
Birch, M.C. 1984. Aggregation in bark beetles, pp. 331–353,in W.J. Bell and R.T. Cardé (eds.). Chemical Ecology of Insects. Sinauer Associates, Sunderland, Massachusetts.
Birch, M.C., andSvihra, P. 1979. Novel approaches to forest insect control: Exploiting olfactory interactions between species of Scolytidae, pp. 135–139,in W.E. Walters (ed.). Current Topics in Forest Entomology. John Wiley & Sons, New York.
Birch, M.C., Svihra, P., Paine, T.D., andMiller, J.C. 1980. Influence of chemically mediated behavior on host tree colonization by four cohabiting species of bark beetles.J. Chem. Ecol. 6:395–414.
Boeckh, J., Kaissling, K.E., andSchneider, D. 1965. Insect olfactory receptors.Cold Spring Harbor Symp. Quant. Biol. 30:263–280.
Borden, J.H., Hunt, D.W.A., Miller, D.R., andSlessor, K.N. 1986. Orientation in forest Coleoptera: An uncertain outcome of responses by individual beetles to variable stimuli, pp. 97–109,in T.L. Payne, M.C. Birch, and C.E.J. Kennedy (eds.). Mechanisms in Insect Olfaction. Clarendon Press, Oxford, UK.
Browne, L.E. 1972. An emergence cage and refrigerator collector for wood-boring insects and their associates.J. Econ. Entomol. 65:1499–1501.
Byers, J.A. 1983. Sex-specific responses to aggregation pheromone: Regulation of colonization density in the bark beetleIps paraconfusus.J. Chem. Ecol. 9:129–142.
Byers, J.A. 1989. Chemical ecology of bark beetles.Experientia 45:271–283.
Delorme, J.D., andPayne, T.L. 1990. Olfactory responses of the black turpentine beetle,Dendroctonus terebrans (Olivier), to beetle pheromones.J. Chem. Ecol. 16:1321–1329.
Dickens, J.C. 1979. Electrophysiological investigations of olfaction in bark beetles.Mitt. Schweiz. Entomol. Ges. 52:203–216.
Dickens, J.C., andPayne, T.L. 1977. Bark beetle olfaction: Pheromone receptor system inDendroctonus frontalis.J. Insect Physiol. 23:481–489.
Dixon, W.N., andPayne, T.L. 1979. Sequence of arrival and spatial distribution of entomophagous and associate insects of southern pine beetle-infested trees. Texas Agriculture Experiment Station Miscellaneous Publication 1432, 28 pp.
Dixon, W.N., andPayne, T.L. 1980. Attraction of entomophagous and associate insects of the southern pine beetle to beetle- and host tree-produced volatiles.J. Ga. Entomol. Soc. 15:378–389.
Godbee, J.F., Jr., andFranklin, R.T. 1976. Attraction, attack patterns and seasonal activity of the black turpentine beetle.Ann. Entomol. Soc. Am. 69:653–655.
Hughes, P.R. 1974. Myrcene: A precursor of pheromones inIps beetles.J. Insect Physiol. 20:1271–1275.
Kinzer, G.W., Fentiman, A.F., Jr., Page, T.F., Jr., Foltz, R.L., Vité, J.P., andPitman, G.B. 1969. Bark beetle attractants: Identification, synthesis and field bioassay of a new compound isolated fromDendroctonus.Nature 221:477–478.
Lanier, G.N. 1983. Integration of visual stimuli, host odorants, and pheromones by bark beetles and weevils in locating and colonizing host trees, pp. 161–171,in S. Ahmad (ed.). Herbivorous Insects: Host Selecting Behavior and Mechanisms. Academic Press, New York.
Light, D.M., andBirch, M.C. 1979. Bark beetle enantiomeric chemoreception: Greater sensitivity to allomone than pheromone.Naturwissenschaften 69:243–245.
Mustaparta, H. 1975. Behavioral responses of the pine weevilHylobius abietis L. (Col.: Curculionidae) to odours activating different groups of receptor cells.J. Comp. Physiol. 102:57–63.
Mustaparta, H., Angst, M.E., andLanier, G.N. 1977. Responses of single cells in the pine engraver beetle,Ips pini (Say) (Coleoptera: Scolitidae) to its aggregation pheromone, ipsdienol, and the aggregation inhibitor, ipsenol.J. Comp. Physiol. 121:343–347.
Mustaparta, H., Angst, M.E., andLanier, G.N. 1979. Specialization of olfactory cells to insect-and host-produced volatiles in the bark beetleIps pini (Say).J. Chem. Ecol. 5:109–123.
Mustaparta, H., Angst, M.E., andLanier, G.N. 1980. Receptor discrimination of enantiomers of the aggregation pheromone ipsdienol, in two species ofIps.J. Chem. Ecol. 6:689–701.
Paine, T.D., Birch, M.C., andSvihra, P. 1981. Niche breadth and resource partitioning by four sympatric species of bark beetles (Coleoptera: Scolytidae).Oecologia 48:1–6.
Payne, T.L., andDickens, J.C. 1976. Adaptation to determine receptor specificity in insect olfactory communication.J. Insect Physiol. 22:1569–1572.
Payne, T.L., Coster, J.E., Richerson, J.V., Edson, L.J., andHart, E.R. 1978. Field response of the southern pine beetle to behavioral chemicals.Environ. Entomol. 7:578–582.
Payne, T.L., Richerson, J.V., Dickens, J.C., West, J.R., Mori, K., Berisford, C.W., Hedden, R.L., Vité, J.P., andBlum, M.S. 1982. Southern pine beetle: Olfactory receptor and behavior discrimination of enantiomers of the attractant pheromone frontalin.J. Chem. Ecol. 8:873–881.
Phillips, T.W., Nation, J.L., Wilkinson, R.C., andFoltz, J.L. 1989. Secondary attraction and field activity of beetle-produced volatiles inDendroctonus terebrans.J. Chem. Ecol. 15:1513–1533.
Quade, D. 1966. On analysis of variance for the k-sample problem.Ann. Math. Stat. 37:1747–1753.
Renwick, J.A.A. 1967. Identification of two oxygenated terpenes from the bark beetlesDendroctonus frontalis andDendroctonus brevicomis.Contrib. Boyce Thompson Inst. 23:355–360.
Renwick, J.A.A., andVité, J.P. 1972. Pheromones and host volatiles that govern aggregation of the six-spined engraver beetle,Ips calligraphus.J. Insect Physiol. 18:1215–1219.
Richerson, J.V., andPayne, T.L. 1979. Effects of bark beetle inhibitors on landing and attack behavior of the southern pine beetle and beetle associates.Environ. Entomol. 8:360–364.
Schneider, D. 1969. Insect olfaction: Deciphering system for chemical messages.Science 163:1031–1037.
Silverstein, R.M., Rodin, J.O., andWood, D.L. 1966. Methodology for isolation and identification of insect pheromones with reference to studies on California five-spinedIps.J. Econ. Entomol. 60:944–949.
Smith, M.T., Busch, G.R., Payne, T.L., andDickens, J.C. 1988. Antennal olfactory responsiveness of three sympatricIps species [Ips avulsus (Eichhoff),Ips calligraphus (Germar),Ips grandicollis (Eichhoff)], to intra- and interspecific behavioral chemicals.J. Chem. Ecol. 14:1289–1304.
Smith, M.T., Payne, T.L., andBirch, M.C. 1990. Olfactory-based behavioral interactions among five species in the southern pine bark beetle group.J. Chem. Ecol. 16:3317–3331.
Svihra, P. 1982. Influence of opposite sex on attraction produced by pioneer sex of four bark beetle species cohabitating pine in the southern United States.J. Chem. Ecol. 8:373–378.
Svihra, P., Paine, T.D., andBirch, M.C. 1980. Interspecific olfactory communications in southern pine beetles.Naturwissenschaften 67:518–519.
Thatcher, R.C.,Searcy, J.L.,Coster, J.E., andHertel, G.D. (eds.). 1980. The Southern Pine Beetle. USDA Forest Service Technical Bulletin 1631, 267 pp.
Tømmerås, B.Å, Mustaparta, H., andGregoire, J.C. 1984. Receptor cells inIps typographus andDendroctonus micans specific to pheromones of the reciprocal genus.J. Chem. Ecol. 10:759–770.
Vité, J.P., andRenwick, J.A.A. 1971a. Inhibition ofDendroctonus frontalis response to frontalin by isomers of brevicomin.Naturwissenschaften 58:418.
Vité, J.P., andRenwick, J.A.A. 1971b. Population aggregating pheromone in the bark beetle,Ips grandicollis.J. Insect Physiol. 17:1699–1704.
Vité, J.P., Gara, R.I., andVon Scheller, H.D. 1964. Field observations on the response to attractants of bark beetles infesting southern pines.Contrib. Boyce Thomp. Inst. 22:461–470.
Vité, J.P., Bakke, A., andRenwick, J.A.A. 1972. Pheromones inIps (Coleoptera: Scolytidae): Occurrence and production.Can. Entomol. 104:1967–1975.
Wadlow, U. 1973. Electrophysiology of a new carrion receptor and its relation to the behavior of the carrion beetle (Necrophorus).J. Comp. Physiol. 83:415–424.
Werner, R.A. 1972a. Aggregation behaviour of the beetleIps grandicollis in response to host-produced attractants.J. Insect Physiol. 18:423–437.
Werner, R.A. 1972b. Response of the beetle,Ips grandicollis, to combinations of host and insect produced attractants.J. Insect Physiol. 18:1403–1412.
Wood, D.L. 1982. The role of pheromones, kairomones, and alomones in the host selection and colonization behavior of bark beetles.Annu. Rev. Entomol. 27:411–446.
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Ascoli-Christensen, A., Salom, S.M. & Payne, T.L. Olfactory receptor cell responses ofIps grandicollis (eichhoff) (Coleoptera: Scolytidae) to intra- and interspecific behavioral chemicals. J Chem Ecol 19, 699–712 (1993). https://doi.org/10.1007/BF00985002
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DOI: https://doi.org/10.1007/BF00985002