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Action ofl-acetylcarnitine on age-dependent modifications of mitochondrial membrane proteins from rat cerebellum

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Abstract

Protein patterns of mitochondrial outer membrane, inner membrane, and matrix from nonsynaptic (free) mitochondria from rat cerebellum at different ages (4, 8, 12, 16, 20, and 24 months) were analyzed by gel electrophoresis. Acutel-acetylcarnitine treatment was per-formed by a single i.p. injection (100 mg/kg body weight) of the substance 60 min before the sacrifice of the animals. Different age-dependent changes were obtained for the proteins of the three fractions. The amount of some protein subunits increased and/or decreased after drug treatment. In particular, protein composition of the inner mitochondrial membrane showed significant age-related modifications. This result probably indicates differences in protein synthesis and/or turnover rates in the various mitochondrial compartments during aging. Acutel-acetylcarnitine treatment caused: a high increase in the amount of one inner membrane protein with Mw 16 kDa, at all the ages studied; a decrease in the amount of many other inner membrane proteins; modifications of some matrix proteins. Our results show that in vivo administration ofl-acetylcarnitine affects mainly the inner membrane protein composition of cerebellar mitochondria.

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Authors and Affiliations

  1. Institute of Pharmacology, Faculty of Science, University of Pavia, Piazza Botta 11, 27100, Pavia, Italy

    R. F. Villa & G. Benzi

  2. Department of Biochemistry, University of Oulu, Oulu, Finland

    L. Turpeenoja

  3. Institute of Biochemistry, Faculty of Medicine, University of Catania, Catania, Italy

    A. M. Giuffrida Stella

Authors
  1. R. F. Villa
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  2. L. Turpeenoja
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  3. G. Benzi
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  4. A. M. Giuffrida Stella
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Villa, R.F., Turpeenoja, L., Benzi, G. et al. Action ofl-acetylcarnitine on age-dependent modifications of mitochondrial membrane proteins from rat cerebellum. Neurochem Res 13, 909–916 (1988). https://doi.org/10.1007/BF00970761

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  • DOI: https://doi.org/10.1007/BF00970761

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