Summary
The importance of a mucus layer in the transepithelial movement of ions was examined in frogs by altering the surface charge density while measuring22Na and36Cl fluxes. Cationic dyes such as Alcian Blue and Ruthenium Red titrate surface mucus but did not affect22Na or36Cl influx or efflux. Other agents such as lanthanum, Azure II, calcium and arginine titrate the subcorni-fied space mucus yet did not affect22Na movement through this action. Thus, the mucus layer does not play a role in epithelial sodium or chloride movement across frog skin.
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Stith, B.J. Effects of an external charged layer on transepithelial ion movement in frog skin. J Comp Physiol B 155, 97–101 (1984). https://doi.org/10.1007/BF00688797
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DOI: https://doi.org/10.1007/BF00688797