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Differentiation of granulocytes in pappenheim stained blood cell smears using standardized cytophotometric analysis

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Summary

The well-known problems of the low reproducibility of peripheral blood smear analysis have for some time stimulated endeavours to automate blood cell classification. In the cytophotometric standardized color measurement and analysis, the computed color characteristics for the first time refer to an internationally accepted color system, allowing not only an international comparison of the computer color measurements but an unproblematic mutual interchange of color information between man and machine based on both the human visual color impressions and the conventional morphological color attributes of the white blood cells. The discriminatory power of the method is demonstrated by differentiating the cytoplasm granulations in basophil, eosinophil and neutrophil granulocytes.

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Authors and Affiliations

  1. Softlab GmbH, Arabella Str. 13, D-8000, München 81, Federal Republic of Germany

    Arnd Rüter

  2. Department of Hematology, Medizinische Universitätsklinik, Josef-Schneider-Str. 2, D-8700, Würzburg, Federal Republic of Germany

    Ulrich Gunzer

Authors
  1. Arnd Rüter
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  2. Ulrich Gunzer
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Additional information

This work was supported by the Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 105 Würzburg) and by the Bundesministerium für Forschung und Technologie

This investigation is part of A. Rüter's thesis at the University of Bremen and was carried out in the SFB 105 Biomedical Image Processing Research Laboratory, University of Virology and Immunbiology, Versbacher Str. 7, D-8700 Würzburg, Federal Republic of Germany

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Rüter, A., Gunzer, U. Differentiation of granulocytes in pappenheim stained blood cell smears using standardized cytophotometric analysis. Blut 48, 307–320 (1984). https://doi.org/10.1007/BF00320402

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