Abstract
Aspergillus niger secretes a number of enzymes, including proteases, into its culture fluid. The regulation of the two major acidic extracellular proteases, pepA and pepB, was investigated using Northern analyses. Our data suggest that the regulation of pepA and pepB expression occurs predominantly at the level of mRNA content and that, while they are regulated in a similar manner, differences are also clear in their expression. Both genes were found to be under complex regulatory control. The expression of the two genes could be turned off by the presence of good nitrogen or carbon sources in the media, and external protein sources did not induce expression of either gene under conditions of carbon and nitrogen repression. The pH of the medium also played a major role in their regulation as the expression of both genes was completely turned off under alkaline conditions, even when grown in media lacking good nitrogen and carbon sources but containing proteins. We isolated clones containing 5′ non-coding sequences of the pepA gene from a λ genomic library with a pepA specific probe. Analysis and comparison of the promoter sequences of the pepA and pepB genes revealed that both contain several putative AREA- and CREA-binding sites and they also share an 18-bp-long sequence which is 83% identical in these two genes.
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Archer DB, Jeenes DJ, MacKenzie DA, Brightwell M, Lambert N, Lowe G, Radford SE, Dobson CM (1990) Bio/Technology 8:741–745
Arst HN, Cove DJ (1973) Mol Gen Genet 216:111–141
Arst HN, MacDonald DW (1975) Nature 254:26–31
Aviv H, Leder P (1972) Proc Natl Acad Sci USA 69:1408–1412
Berka RM, Ward M, Wilson LJ, Hayenga KJ, Kodama KH, Carlomango LP, Thompson SA (1990) Gene 86:153–162
Berka RM, Kodama KH, Rey MW, Wilson LJ, Ward M (1991) Biochem Soc Trans 19:681–685
Caddick MX, Brownlee AG, Arst HN (1986) Mol Gen Genet 203:346–353
Davis MA, Hynes MJ (1991) Regulatory circuits in Aspergillus nidulans. In: Bennett JW, Lasure LL (eds) More gene manipulations in fungi. Academic Press, London, pp 151–189
Dowzer CEA, Kelly JM (1991) Mol Cell Biol 11:5701–5709
Drysdale MR, Kolze SE, Kelly JM (1993) Gene 130:241–245
Dunn-Coleman NS, Bloebaum P, Berka RM, Bodie E, Robinson N, Armstrong G, Ward M, Przetak M, Carter GL, LaCost R, Wilson LJ, Kodama KH, Baliu EF, Bower B, Lamsa M, Heinsohn H (1991) Bio/Technology 9:976–981
Espeso EA, Tilburn J, Arst HN, Peñalva MA (1993) EMBO J 12:3947–3956
Frederick GD, Rombouts P, Buxton FP (1993) Gene 125:57–64
Fu YH, Marzluf GA (1990) Proc Natl Acad Sci USA 87:5331–5335
Goosen T, Bloemheuvel G, Gysler C, Debie DA, Van den Broek HWJ, Swart K (1987) Curr Genet 11:499–503
Graaf L, van den Broeck H, Visser J (1992) Curr Genet 22:21–27
Hanson MA, Marzluf GA (1975) Proc Natl Acad Sci USA 72:1240–1244
Harmsen JAM, Kusters-van Someren MA, Visser J (1990) Curr Genet 18:161–166
Inoue H, Kimura T, Makabe O, Takahashi K (1991) J Biol Chem 266:19484–19489
Jarai G, van den Hombergh H, Buxton F (1994) Gene (in press)
Jeenes DJ, MacKenzie DA, Roberts IN, Archer DB (1991) Biotech Genet Eng Rev 9:327–367
Kudla B, Caddick MX, Langdon T, Martini-Rossi NM, Bennett CF, Sibley S, Davies RW, Arst HN (1990) EMBO J 9:1355–1364
Kulumburg P, Mathieu M, Dowzer C, Kelly JM, Felenbok B (1993) Mol Microbiol 7:847–857
Marzluf GA (1981) Microbiol Rev 45:437–461
Roberts IN, Jeenes DJ, MacKenzie DA, Wilkinson AP, Sumner IG, Archer DB (1992) Gene 122:155–161
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Ward M, Wilson LJ, Kodama KH, Rey MW, Berka RM (1990) Bio/Technology 8:435–440
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Jarai, G., Buxton, F. Nitrogen, carbon, and pH regulation of extracellular acidic proteases of Aspergillus niger . Curr Genet 26, 238–244 (1994). https://doi.org/10.1007/BF00309554
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DOI: https://doi.org/10.1007/BF00309554