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Callus induction and plant regeneration from mature zygotic embryos of a tetraploid Alstroemeria (A. pelegrina × A. psittacina)

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Summary

A simple procedure was developed to induce callus growth and whole plant regeneration for a tetraploid cultivar of Alstroemeria. The callus, induced from mature zygotic embryos cultured on a medium supplemented with 20 μM kinetin with 10 or 20 μM NAA, could be maintained for one year without any loss of regeneration potential. Maximum frequency of regeneration (40%) was obtained with calli maintained on the medium containing 20 μM kinetin and 20 μM NAA. Whole plant regeneration occurred via somatic embryogenesis in the absence of growth regulators and the plantlets grew to maturity and flowered in the greenhouse conditions.

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Abbreviations

BAP:

N6-benzylaminopurine

MS:

Murashige and Skoog (1962) medium

MSO:

Basal medium devoid of any plant growth regulator

NAA:

α-Naphthaleneacetic acid

TDZ:

N-phenyl-N 1,2,3,-thiadiazol-5-ylurea (thidiazuron)

IAA:

Indole-3-acetic acid

2,4-D:

2,4-Dichlorophenoxyacetic acid

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Authors and Affiliations

  1. Department of Horticultural Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Margaret J. Hutchinson, J. M. Tsujita & Praveen K. Saxena

Authors
  1. Margaret J. Hutchinson
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  2. J. M. Tsujita
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  3. Praveen K. Saxena
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Communicated by W. Parrott

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Hutchinson, M.J., Tsujita, J.M. & Saxena, P.K. Callus induction and plant regeneration from mature zygotic embryos of a tetraploid Alstroemeria (A. pelegrina × A. psittacina). Plant Cell Reports 14, 184–187 (1994). https://doi.org/10.1007/BF00233787

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  • DOI: https://doi.org/10.1007/BF00233787

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