Abstract
Homozygous wheat/rye (1BL/1RS or 1AS/ 1RL) translocation lines have significantly contributed to wheat production, and several other wheat/rye translocation lines show a potential promise against biotic and abiotic stresses. Detecting the presence of rye at the chromosome level is feasible by C-banding and isozyme protocols, but the diagnostic strength of genomic in situ hybridization for eventually analyzing smaller DNA introgressions has greater significance. As a first step we have applied the genomic in situ hybridization technique to detect rye chromosomes in a wheat background using germ plasm of agricultural significance. By this method rye contributions to the translocations 1BL/1RS, 1AL/1RS, 5AS/5RL and 6BS/6RL could be identified. Differential labelling has further enabled the detection of rye and Thinopyrum bessarabicum chromosomes in a trigeneric hybrid of Triticum aestivum/Th. bessarabicum//Secale cereale.
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Communicated by K. Tsunewaki
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Islam-Faridi, M.N., Mujeeb-Kazi, A. Visualization of Secale cereale DNA in wheat germ plasm by fluorescent in situ hybridization. Theoret. Appl. Genetics 90, 595–600 (1995). https://doi.org/10.1007/BF00222120
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DOI: https://doi.org/10.1007/BF00222120