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Two components of onset and recovery during photoinhibition of Ulva rotundata

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Abstract

Short-term (up to 5 h) transfers of shade-adapted (100 μmol · m−2 · s−1) clonal tissue of the marine macroalga Ulva rotundata Blid. (Chlorophyta) to higher irradiances (1700, 850, and 350 μmol · m−2 · s−1) led to photoinhibition of room-temperature chlorophyll fluorescence and O2 evolution. The ratio of variable to maximum (Fv/Fm) and variable (Fv) fluorescence, and quantum yield (ϕ) declined with increasing irradiance and duration of exposure. This decline could be resolved into two components, consistent with the separation of photoinhibition into energy-dissipative processes (photoprotection) and damage to photosystem II (PSII) by excess excitation. The first component, a rapid decrease in Fv/Fm and in Fv, corresponds to an increase in initial (Fo) fluorescence and is highly sensitive to 1 mM chloramphenicol. This component is rapidly reversible under dim (40 μmol · m−2 · s−1) light, but is less reversible with increasing duration of exposure, and may reflect damage to PSII. The second (after 1 h exposure) component, a slower decline in Fv/Fm and Fv with declining Fo, appears to be associated with the photoprotective interconversion of violaxanthin to zeaxanthin and is sensitive to dithiothreitol. The accumulation of zeaxanthin in U. rotundata is very slow, and may account for the predominance of increases in Fo at high irradiances.

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Abbreviations

CAP:

chloramphenicol

DTT:

dithiothreitol

Fo, Fm, Fv :

initial, maximum, and variable fluorescence

ϕ:

quantum yield

PFD:

photon flux density

PSII:

photosystem II

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Authors and Affiliations

  1. Duke University Marine Laboratory, NC 28516-9721, Beaufort, USA

    Linda A. Franklin, Guy Levavasseur, C.Barry Osmond, William J. Henley & Joseph Ramus

  2. Department of Botany, Duke University, NC 27706, Durham, USA

    Linda A. Franklin, C.Barry Osmond & Joseph Ramus

  3. Station Biologique, CNRS and Université Paris VI, F-29680, Roscoff, France

    Guy Levavasseur

Authors
  1. Linda A. Franklin
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  2. Guy Levavasseur
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  3. C.Barry Osmond
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  4. William J. Henley
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  5. Joseph Ramus
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To whom correspondence should be addressed

We are grateful to O. Björkman and S. Thayer, Carnegie Institution of Washington, Stanford, Cal., USA, for analysis of xanthophyll pigments reported here. This research was supported by National Science Foundation grant OCE-8812157 to C.B.O. and J.R. Support for G.L. was provided by a NSF-CNRS (Centre National de la Recherche Scientifique) exchange fellowship.

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Franklin, L.A., Levavasseur, G., Osmond, C. et al. Two components of onset and recovery during photoinhibition of Ulva rotundata . Planta 186, 399–408 (1992). https://doi.org/10.1007/BF00195321

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