Abstract
A newly established cell line, designated NRS-1, was derived from an alveolar rhabdomyosarcoma that developed in the left forearm of a 7-year-old girl. The cell line had a t(2; 13) chromosomal translocation. Reverse transcription-polymerase chain reaction demonstrated that 5′ PAX3-3′ FKHR chimeric transcript was expressed in NRS-1 cells. NRS-1 cells showed myogenic differentiation without any particular stimulus in vitro and exhibited various kinds of muscle markers. All-trans retinoic acid promoted cell differentiation in the myogenic direction. Transforming growth factor-β (TGF-β) inhibited myogenic differentiation of those cells and promoted cell proliferation.
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Ogose, A., Motoyama, T., Watanabe, H. et al. In vitro differentiation and proliferation in a newly established human rhabdomyosarcoma cell line. Vichows Archiv A Pathol Anat 426, 385–391 (1995). https://doi.org/10.1007/BF00191348
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DOI: https://doi.org/10.1007/BF00191348