Summary
An alkaline protease, previously isolated from a symbiotic bacterium found in the gland of Deshayes of marine shipworm, was evaluated as a cleansing additive. The protease nearly doubled the cleaning power of a standard phosphate detergent at temperatures up to 50°C as determined by fabric swatch assays. The enzyme was, however, ineffective at 70°C. In both fresh and seawater, it was also an efficient presoak. The cleaning power of a non-phosphate detergent was significantly improved by added protease, independent of the pH range 10 to 12. The enzyme degraded lysozyme, the major protein contaminant of contact lenses, more extensively than subtilisin and was effective in solutions containing hydrogen peroxide, often employed to sterilize lenses. The protease was unusually stable in sodium perborate, as well as hydrogen peroxide, and retained good activity in the presence of sodium hypochloride.
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Greene, R.V., Griffin, H.L. & Cotta, M.A. Utility of alkaline protease from marine shipworm bacterium in industrial cleansing applications. Biotechnol Lett 18, 759–764 (1996). https://doi.org/10.1007/BF00127884
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DOI: https://doi.org/10.1007/BF00127884