Skip to main content
Log in

The minimum dietary requirement of vitamin C in Atlantic salmon (Salmo salar) fry using Ca ascorbate-2-monophosphate as dietary source

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

The minimum dietary vitamin C requirement for optimal growth and normal development in Atlantic salmon (Salmo salar) fry at the onset of feeding was studied, using Ca ascorbate-2-monophosphate (AP) as dietary source. The requirement was established by means of a feeding study lasting for 23 weeks from the beginning of feeding. The practical diets used were supplemented with AP at levels of 0, 10, 20, 40, 80 and 160 mg ascorbic acid (AA) equivalents/kg. Growth, mortality, hydroxyproline content in skin and backbone, and AA in liver were recorded to evaluate the results. The results suggest that the minimum dietary requirement for optimal growth and normal development is in the range of 10–20 mg AA equivalents/kg dry diet during the period studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References cited

  • Albrektsen, S., Lie, Ø. and Sandnes, K. 1988. Ascorbyl palmitate as a dietary vitamin C source for rainbow trout (Salmo gairdneri). Aquaculture 71: 359–368.

    Google Scholar 

  • Barnes, M.J. and Kodicek, E. 1972. Biological hydroxylations and ascorbic acid with special regard to collagen metabolism. Vit. Horm. 30: 1–43.

    Google Scholar 

  • Chavez de Martinez, M.C. 1990. Vitamin C requirement of the mexican native chichlid Cichlasoma urphthalmus (Gunther). Aquaculture 86: 409–416.

    Google Scholar 

  • Cowey C.B. 1988. The Nutrition of fish: The developing scene. Nutr. Res. Rev. 1: 255–280.

    Google Scholar 

  • Durve, V.S. and Lovell, R.T. 1982. Vitamin C and disease resistance in channel catfish (Ictalurus punctatus). Can. J. Fish. Aquat. Sci. 39: 948–951.

    Google Scholar 

  • Halver, J.E., Ashley, L.M. and Smith, R.R. 1969. Ascorbic acid requirements of coho salmon and rainbow trout. Trans. Am. Fish. Soc. 98: 762–771.

    Google Scholar 

  • Halver, J.E., Smith, R.R., Tolbert, B.M. and Baker, E.M. 1975. Utilization of ascorbic acid in fish. Ann. N.Y. Acad. Sci. 258: 81–102.

    Google Scholar 

  • Halver, J.E. 1982. The vitamins required for cultivated salmonids. Comp. Biochem. Physiol. 73B: 43–50.

    Google Scholar 

  • Hardie, L.J., Fletcher, T.C. and Secombes, C.J. 1991. The effect of dietary vitamin C on the immune response in Atlantic salmon (Salmo salar). Aquaculture 95: 201–214.

    Google Scholar 

  • Hilton, J.W., Cho, C.Y. and Slinger, S.J. 1977a. Factors affecting the stability of supplemental ascorbic acid in practical trout diets. J. Fish. Res. Bd. Can. 34: 683–687.

    Google Scholar 

  • Hilton, J.W., Cho, C.Y. and Slinger, S.J. 1977b. Evaluation of the ascorbic acid status of rainbow trout (Salmo gairdneri). J. Fish. Res. Bd. Can. 34: 2207–2210.

    Google Scholar 

  • Hilton, J.W., Cho,, C.Y. and Slinger, S.J. 1978. Effect of graded levels of supplemental ascorbic acid in practical diets fed to rainbow trout (Salmo gairdneri). J. Fish. Res. Bd. Can. 35: 431–436.

    Google Scholar 

  • Hilton, J.W. 1989. The interaction of vitamins, minerals and diet composition in the diet of fish. Aquaculture 79: 223–244.

    Google Scholar 

  • John, T.M., George, J.C., Hilton, J.W. and Slinger, S.J. 1979. Influence of dietary ascorbic acid on plasma lipid levels in the rainbow trout. Int. J. Vit. Nutr. Res. 49: 400–405.

    Google Scholar 

  • Johnston, W.L., MacDonald, E. and Hilton, J.W. 1989. Relationships between dietary ascorbic acid status and deficiency, weight gain and brain neurotransmitter levels in juvenile rainbow trout, Salmo gairdneri. Fish Physiol. Biochem. 6: 353–365.

    Google Scholar 

  • Maage, A., Waagbø, R., Olsson, P.E., Julshamn, K. and Sandnes, K. 1990. Ascorbate-2-sulfate as a dietary vitamin C source for Atlantic salmon (Salmo salar): 2. Effects of dietary levels and immunization on the metabolism of trace elements. Fish Physiol. Biochem. 8: 429–436.

    Google Scholar 

  • Mahajan, C.L. and Agrawal, N.K. 1980. Nutritional requirement of ascorbic acid by indian major carp, Cirrhina Mrigala, during early growth. Aquaculture 19: 37–48.

    Google Scholar 

  • Navarre, O. and Halver, J.E. 1989. Disease resistance and humoral antibody production in rainbow trout fed high levels of vitamin C. Aquaculture 79: 207. /221.

    Google Scholar 

  • NRC 1981. Nutrient requirements of coldwater fishes, 16. National Academy Press, Washington, D.C.

    Google Scholar 

  • Padh, H. 1991. Vitamin C: Newer insights into its biochemical functions. Nutr. Rev. 49: 65–70.

    Google Scholar 

  • Pang, P.K.T. 1971. The effects of complete darkness and vitamin C supplement on the killfish, Fundulus heteroclitus, adapted to sea water. J. Exp. Zool. 178: 15–22.

    Google Scholar 

  • Roy, R.B., Conetta, A. and Salpeter, J. 1976. Automated fluorometric method for the determination of total vitamin C in food products. J. Assoc. Off. Anal. Chem. 59: 1244–1250.

    Google Scholar 

  • Sandnes, K. 1982. Vitamin C in aquaculture. Analysis. Stability in dry feed. Requirement in rainbow trout (Salmo gairdneri) (in Norwegian). Cand. Real. Thesis, University of Bergen, Norway.

  • Sandnes, K., Hansen, T., Killie, J-E.A. and Waagbø, R. 1990. Ascorbate-2-sulfate as a dietary vitamin C source for Atlantic salmon (Salmo salar): 1. Growth, bioactivity, haematology and humoral immune response. Fish Physiol. Biochem. 8: 419–427.

    Google Scholar 

  • Sandnes, K. Ulgenes, Y., Braekkan, O.R. and Utne, F. 1984. The effect of ascorbic acid supplementation in broodstock feed on reproduction of rainbow trout (Salmo gairdneri). Aquaculture 43: 167–177.

    Google Scholar 

  • Sandnes, K. and Utne, F. 1982. Processing loss and storage stability of ascorbic acid in dry fish feed. Fisk. Dir. Skr. Ser. Ernæring 2: 39–44.

    Google Scholar 

  • Sandnes, K. and Waagbø, R. 1991. Enzymatic hydrolysis of ascorbate-2-monophosphate and ascorbate-2-sulfate in vitro, and bioactivity of ascorbate-2-monophosphate in Atlantic salmon (Salmo salar). Fisk. Dir. Skr. Ser. Ernæring 4: 33–39.

    Google Scholar 

  • Sato, M. Yoshinaka, R. and Ikeda, S. 1978. Dietary ascorbic acid requirement of rainbow trout for growth and collagen formation. Bull. Jap. Soc. Sci. Fish. 44: 1029–1035.

    Google Scholar 

  • Sato, M., Kondo, T., Yoshinaka, R. and Ikeda, S. 1982. Effect of dietary ascorbic acid levels on collagen formation in rainbow trout. Bull. Jap. Soc. Sci. Fish. 48: 553–556.

    Google Scholar 

  • Shigueno, K. and Itoh, S. 1988. Use of Mg-ascorbyl-2-phosphate as a vitamin C source in shrimp diets. J. World Aquacult. Soc. 19: 168–174.

    Google Scholar 

  • Slinger, S.J., Razzaque, A. and Cho, C.Y. 1979. Effect of feed processing and leaching on the losses of certain vitamins in fish diets. Proc. World Symp. on Finfish Nutrition and Fishfeed Technology, Vol. II: 425–434.

    Google Scholar 

  • Soliman, A.K., Jauncey, K. and Roberts, R.J. 1986. The effects of dietary ascorbic acid supplementation on hatachability, survival rate and fry performance in Oreochromis mossambicus (Peters). Aquaculture 59: 197–208.

    Google Scholar 

  • Thomas, P. and Neff, J.M. 1984. Effects of a pollutant and other environmental variables on the ascorbic acid content of fish tissues. Mar. Environ. Res. 14: 489–491.

    Google Scholar 

  • Waagbø, R., Thorsen, T. and Sandnes, K. 1989. Role of dietary ascorbic acid in vitellogenesis in rainbow trout (Salmo gairdneri). Aquaculture 80: 301–314.

    Google Scholar 

  • Wedemeyer, G. 1969. Stress-induced ascorbic acid depletion and cortisol production in two salmonid fishes. Comp. Biochem. Physiol. 29: 1247–1251.

    Google Scholar 

  • Wilson, R.P. and Poe, W. 1973. Impaired collagen formation in the scorbutic channel catfish, J. Nutr. 103: 1359–1364.

    Google Scholar 

  • Yoshinaka, R., Sato, M. and Ikeda, S. 1978. In vitro formation of collagen in skin of ascorbic acid deficient rainbow trout. Bull. Jap. Soc. Sci. Fish. 44: 1147–1150.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sandnes, K., Torrissen, O. & Waagbø, R. The minimum dietary requirement of vitamin C in Atlantic salmon (Salmo salar) fry using Ca ascorbate-2-monophosphate as dietary source. Fish Physiol Biochem 10, 315–319 (1992). https://doi.org/10.1007/BF00004480

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00004480

Keywords

Navigation