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Kluyveromyces lactis zymocin and other plasmid-encoded yeast killer toxins

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Microbial Protein Toxins

Part of the book series: Topics in Current Genetics ((TCG,volume 11))

Abstract

Supported by an endogenous killer plasmid DNA system that codes for an anti-proliferative zymocin toxin, the dairy yeast Kluyveromyces lactis has attracted research into inter-yeast pathogenesis. Molecularly, gene disruption and shuffle techniques have proven powerful means to study components essentially required for the maintenance of the killer plasmid system, namely, autonomously operating replication and transcription apparatuses. Moreover, by studying zymocin action towards sensitive Saccharomyces cerevisiae cells, a multi-step response has surfaced that involves chitin docking, import and communication of zymocin’s γ-toxin with Elongator’s toxin-target (TOT) function and eventually, a terminal G1 block. Though apparently different with respect to toxin-target strategies, novel yeast killers that are phylogenetically related to the K. lactis system have shown to establish cell surface contact by zymocin-like chitin binding. Hence, research into zymocin as a model may provide new insights into evolution of yeast pathosystems and proliferation control by toxins.

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Authors and Affiliations

  1. Biologicum, Institut für Genetik, Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 10, D-06120 Halle (Saale), Germany

    Raffael Schaffrath

  2. Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstr. 3, D-48149 Münster, Germany

    Friedhelm Meinhardt

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  1. Raffael Schaffrath
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Correspondence to Raffael Schaffrath .

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Manfred J. Schmitt Raffael Schaffrath

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Schaffrath, R., Meinhardt, F. Kluyveromyces lactis zymocin and other plasmid-encoded yeast killer toxins. In: Schmitt, M.J., Schaffrath, R. (eds) Microbial Protein Toxins. Topics in Current Genetics, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b100196

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