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The rise of multidrug resistant Salmonella isolates in healthy chickens in Brazil by successful establishment of plasmid IncHI2A carrying several antibiotic resistance genes

  • Veterinary Microbiology - Research Paper
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Abstract

Salmonella spp. is an important global issue in food-producing animals. The present study evaluated antimicrobial resistance and virulence profiles in Salmonella spp. isolates from chickens in Brazil. Identification of serotypes, virulence and antimicrobial resistance genes, and plasmid profiles were performed. Three different serovars were found, S. Schwarzengrund, S. Newport and S. Kentucky. All isolates were considered Multidrug- resistance (MDR). Among the 32 Salmonella spp. isolates analysed, 29 isolates carried blaCTX-M-2 gene and showed the insertion sequence ISCR1 and a class 1 integron structure upstream from blaCTX-M-2. This gene was harboured in large IncHI2A plasmids with approximately 280kb. Furthermore, 30 isolates harboured tetA and tetB genes and 25 also harboured qnrB. The virulence genes invA, misL, orfL, spiC and pipD were detected in all isolates. The study shows a high prevalence of MDR Salmonella isolates disseminated in poultry farms. The association of the replicon IncHI2A with the resistance genes found, elevate the risk of foodborne disease outbreaks.

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Acknowledgements

We would like to thank Dr. Luke Richards for his kind review of the text. São Paulo Research Foundation (FAPESP) for the constant support for our research (Grant n. 2014/14494-8). Rafael. Penha Filho, (FAPESP, grant 2017/00620-0).

Funding

This study was funded by São Paulo Research Foundation (FAPESP), Grant n. 2014/14494-8.

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Authors and Affiliations

  1. Department of Veterinary Pathology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP, 14884-900, Brazil

    Rafael Antonio Casarin Penha Filho & Angelo Berchieri Jr.

  2. School of Pharmaceutical Sciences of Ribeirão Preto – University of São Paulo (USP), Ribeirão Preto, SP, 14040-903, Brazil

    Joseane Cristina Ferreira, Renata Galetti & Ana Lúcia da Costa Darini

  3. Instituto Biológico de Descalvado, Descalvado, SP, 13690-000, Brazil

    Ana Maria Iba Kanashiro

Authors
  1. Rafael Antonio Casarin Penha Filho
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  2. Joseane Cristina Ferreira
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  3. Renata Galetti
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  4. Ana Maria Iba Kanashiro
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  5. Angelo Berchieri Jr.
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  6. Ana Lúcia da Costa Darini
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Contributions

R.A.C.P.F. and J.C.F. carried out the experiment and wrote the manuscript with support from A.B.Jr. and A.L.C.D, R.G. contributed to the interpretation of the results and A. M. I. K. contributed to sample preparation and isolation of samples. All authors contributed to the final version of the manuscript. A.L.C.D supervised the project.

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Correspondence to Rafael Antonio Casarin Penha Filho.

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Penha Filho, R.A.C., Ferreira, J.C., Galetti, R. et al. The rise of multidrug resistant Salmonella isolates in healthy chickens in Brazil by successful establishment of plasmid IncHI2A carrying several antibiotic resistance genes. Braz J Microbiol 54, 469–474 (2023). https://doi.org/10.1007/s42770-022-00893-0

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  • DOI: https://doi.org/10.1007/s42770-022-00893-0

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