Abstract
On juveniles of Oreochromis niloticus, the protective potential of ascorbic acid (Asc) against oxidative stress and genotoxicity induced by acetamiprid (Aceta) sub-lethal concentrations was investigated in this study. Fishes were divided into six groups and exposed to either Asc (50 ppm), 10 and 20 ppm Aceta, 10 ppm (Aceta)+Asc, 20 ppm (Aceta)+Asc, or the unexposed control group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities and their transcripts were assessed. DNA damage in erythrocytes, hepatocytes, and gill cells, in addition to the mitotic index (MI), and the existence of erythrocytic nuclear abnormalities (ENAs) were performed. The results showed that concentrations of Aceta (10 and 20 ppm) induced oxidative stress by altering the antioxidant enzyme activities and transcripts. There were genotoxic effects of Aceta exposure showed by the significant (P < 0.05) increase in DNA-damaged cells and ENA, meanwhile a decrease in MI. Co-exposure with Asc showed significant alleviations of oxidative status and genotoxicity. Thus, results suggest that Asc-combined exposure could be the effective treatment against Aceta-induced oxidative stress accompanied with genotoxicity in O. niloticus.
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I.E-G. and H.S. conceived and supervised the study concept. H.H. and I.E-G. performed the practical part of the study. H.H, S.A, and I.E-G. wrote the manuscript draft. I.E-G. and H.S. revised the final version of the manuscript. H.H., I.E-G., S.A., and H.S. read and approved the final manuscript.
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Hathout, H.M.R., Sobhy, H.M., Abou-Ghanima, S. et al. Ameliorative role of ascorbic acid on the oxidative stress and genotoxicity induced by acetamiprid in Nile tilapia (Oreochromis niloticus). Environ Sci Pollut Res 28, 55089–55101 (2021). https://doi.org/10.1007/s11356-021-14856-9
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DOI: https://doi.org/10.1007/s11356-021-14856-9