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Effect of carbon source on lipid accumulation and biodiesel production of Yarrowia lipolytica

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Abstract

Yarrowia lipolytica (Y. lipolytica) is an oleaginous yeast that can utilize hydrophobic substrates as carbon source to produce single-cell lipids for biodiesel production. This study attempts to increase the lipid accumulation ability of Y. lipolytica by first gradually elevating pure oil substrate concentration during the cultivation and then adding short-chain carbon compounds, such as glucose and sodium acetate, to a culture substance according to the optimal oil concentration. Results showed that Y. lipolytica cultured under 40.0 g L−1 oil concentration showed higher lipids (2.97 g L−1) and lipid content (37.35%, DW) compared with that cultured under 20.0 g L−1, where the corresponding values were 1.91 g L−1 and 24.46%. By contrast, the lipid content of Y. lipolytica increased from 37.35 to 41.50% when the substrate was changed from 40.0 g L−1 pure oil to 5% sodium acetate combined with 95% oil under the same total carbon concentration. However, lipid accumulation did not increase even though 15% sodium acetate or 5% glucose, or 15% glucose was added to the combined substrate. Moreover, the lipid biomodification of Y. lipolytica was evident when it was cultured under the oil concentration of 20.0 g L−1. Therefore, the lipid accumulation of Y. lipolytica can be elevated through the gradient increase of oil concentration and by adding a suitable amount of easily degradable carbon source. Furthermore, the lipid biomodification of Y. lipolytica improves biodiesel quality.

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References

  • Belle HV, Goossens F (2011) Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production. Eur J Lipid Sci Technol 113:1031–1051

    Article  Google Scholar 

  • Chen YC, Lin DY, Chen BH (2017) Transesterification of acid soybean oil for biodiesel production using lithium metasilicate catalyst prepared from diatomite. J Taiwan Inst Chem Eng 79:31–36

    Article  CAS  Google Scholar 

  • Donot F, Fontana A, Baccou JC, Strub C, Schorr-Galindo S (2014) Single cell oils (SCOs) from oleaginous yeasts and moulds: production and genetics. Biomass Bioenergy 68:135–150

    Article  CAS  Google Scholar 

  • Dumore NS, Mukhopadhyay M (2012) Removal of oil and grease using immobilized triacylglycerin lipase. Int Biodeterior Biodegrad 68:65–70

    Article  CAS  Google Scholar 

  • Etchepare R, Oliveira H, Azevedo A, Rubio J (2017) Separation of emulsified crude oil in saline water by dissolved air flotation with micro and nanobubbles. Curr Microbiol 186:326–332

    CAS  Google Scholar 

  • Fickers P, Nicaud JM, Destain J, Thonart P (2005) Involvement of hexokinase Hxk1 in glucose catabolite repression of LIP2 encoding extracellular lipase in the yeast Yarrowia lipolytica. Curr Microbiol 50:133–137

    CAS  Google Scholar 

  • Katre G, Ajmera N, Zinjarde S, Ravikumar A (2017) Mutants of Yarrowia lipolytica NCIM 3589 grown on waste cooking oil as a biofactory for biodiesel production. Microb Cell Factories 16:176–188

    Article  Google Scholar 

  • Li Y, Mei H, Fang H (2013) A review of treating oily wastewater. Arab J Chem 265:1913–1922

    Google Scholar 

  • Liu HH, Ji XJ, Huang H (2015) Biotechnological applications of Yarrowia lipolytica: past, present and future. Biotechnol Adv 33:1522–1546

    Article  CAS  Google Scholar 

  • Ma X, Gao M, Gao Z, Wang J, Zhang M, Ma Y, Wang Q (2018a) Past, current, and future research on microalga-derived biodiesel: a critical review and bibliometric analysis. Environ Sci Pollut Res 25:1–15

    Google Scholar 

  • Ma Y, Gao Z, Wang Q, Liu Y (2018b) Biodiesels from microbial oils: opportunity and challenges. Bioresour Technol 263:631–641

    Article  CAS  Google Scholar 

  • Pandit PR, Fulekar MH, Karuna MSL (2017) Effect of salinity stress on growth, lipid productivity, fatty acid composition, and biodiesel properties in Acutodesmus obliquus and Chlorella vulgaris. Environ Sci Pollut Res 24:13437–13451

    Article  CAS  Google Scholar 

  • Papanikolaou S, Chevalot I, Galiotou-Panayotou M, Komaitis M, Marc I, Aggelis G (2007) Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica. Electron J Biotechnol 10:425–435

    Article  CAS  Google Scholar 

  • Papanikolaou S, Aggelis G (2010) Selective uptake of fatty acids by the yeast Yarrowia lipolytica. Eur J Lipid Sci Technol 105:651–655

    Article  Google Scholar 

  • Putatunda S, Bhattacharya S, Sen D, Bhattacharjee C (2018) A review on the application of different treatment processes for emulsified oily wastewater. Int J Environ Sci Technol 16:2525–2536

    Article  Google Scholar 

  • Qin L, Wang Z, Sun Y, Shu Q, Feng P, Zhu L, Xu J, Yuan Z (2016) Microalgae consortia cultivation in dairy wastewater to improve the potential of nutrient removal and biodiesel feedstock production. Environ Sci Pollut Res 23:8379–8387

    Article  CAS  Google Scholar 

  • Sahar SS, Iqbal J, Ullah I, Bhatti HN, Nouren S, Habib-ur-Rehman NJ, Iqbal M (2018) Biodiesel production from waste cooking oil: an efficient technique to convert waste into biodiesel. Sust Cities Soc 41:220–226

    Article  Google Scholar 

  • Saki S, Uzal N (2018) Preparation and characterization of PSF/PEI/CaCO3 nanocomposite membranes for oil/water separation. Environ Sci Pollut Res 25:1–12

    Article  Google Scholar 

  • Sivaramakrishnan R, Incharoensakdi A (2017) Enhancement of lipid production in Scenedesmus sp. by UV mutagenesis and hydrogen peroxide treatment. Bioresour Technol 235:366–370

    Article  CAS  Google Scholar 

  • Song H, Zhou L, Zhang L, Bei G, Wei D, Shen Y, Rui W, Madzak C, Jiang Z (2011) Construction of a whole-cell catalyst displaying a fungal lipase for effective treatment of oily wastewaters. J Mol Catal B-Enzym 71:166–170

    Article  CAS  Google Scholar 

  • Wang Y, Feng S, Bai X, Zhao J, Xia S (2015) Scum sludge as a potential feedstock for biodiesel production from wastewater treatment plants. Waste Manag 47:91–97

    Article  Google Scholar 

  • Zeng Y, Yang C, Zhang J, Pu W (2007) Feasibility investigation of oily wastewater treatment by combination of zinc and PAM in coagulation/flocculation. J Hazard Mater 147:991–996

    Article  CAS  Google Scholar 

  • Zhang X, Bing Z, Wu Y, Wang T, Qiu J (2018) Preparation and characterization of a diatomite hybrid microfiltration carbon membrane for oily wastewater treatment. J Taiwan Inst Chem Eng 89:39–48

    Article  CAS  Google Scholar 

  • Zhao C, Zheng H, Gao B, Liu Y, Zhai J, Zhang S, Xu B (2018) Ultrasound-initiated synthesis of cationic polyacrylamide for oily wastewater treatment: enhanced interaction between the flocculant and contaminants. Ultrason Sonochem 42:31–41

    Article  CAS  Google Scholar 

  • Živković S, Veljković M (2018) Environmental impacts the of production and use of biodiesel. Environ Sci Pollut Res 25:191–199

    Article  Google Scholar 

Download references

Funding

This work was supported by the National Natural Science Foundation of China (No. 21677115) and the Shaanxi Provincial Natural Science Foundation Research Key Project (No. 2016JZ019).

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Authors and Affiliations

  1. School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an, 710055, China

    Baohua Chai, Yi Wang, Wenhuai Wang & Pan Fan

  2. Shaanxi Key Laboratory of Environmental Engineering, Xi’an University of Architecture and Technology, Xi’an, 710055, People’s Republic of China

    Baohua Chai, Yi Wang, Wenhuai Wang & Pan Fan

  3. Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi’an University of Architecture and Technology, Xi’an, 710055, People’s Republic of China

    Baohua Chai, Yi Wang, Wenhuai Wang & Pan Fan

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  1. Baohua Chai
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  2. Yi Wang
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  3. Wenhuai Wang
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  4. Pan Fan
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Correspondence to Yi Wang.

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Responsible editor: Ta Yeong Wu

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Chai, B., Wang, Y., Wang, W. et al. Effect of carbon source on lipid accumulation and biodiesel production of Yarrowia lipolytica. Environ Sci Pollut Res 26, 31234–31242 (2019). https://doi.org/10.1007/s11356-019-06249-w

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  • DOI: https://doi.org/10.1007/s11356-019-06249-w

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