Abstract
Purpose
The objective of the present study is to determine whether uptake of [18F]fluoromethylcholine ([18F]FCH) in comparison with 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) accurately reflects chemotherapy efficacy at the tumor cell level in prostate cancer (PC).
Procedures
The effects of docetaxel and cabazitaxel on viable tumor cell number were explored in four PC cell lines. Cellular uptake of [18F]FDG and [18F]FCH was compared with the effects measured using sulforhodamine B (SRB) assay, cell counting and colony formation assay (CFA), as proximators of viable tumor cell number. Agreement between uptake and cell numbers was assessed by Bland-Altman plots.
Results
[18F]FCH uptake in all PC cell lines significantly correlated to the cell numbers surviving the respective drug concentrations. Bland-Altman analysis showed that [18F]FDG uptake resulted in signal overestimation and higher variability after chemotherapy.
Conclusions
[18F]FCH uptake correlates well with viable tumor cell numbers remaining after docetaxel and cabazitaxel exposure. Radiolabeled choline is a potential response monitoring biomarker after chemotherapy for PC.
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Oprea-Lager, D.E., van Kanten, M.P., van Moorselaar, R.J.A. et al. [18F]Fluoromethylcholine as a Chemotherapy Response Read-Out in Prostate Cancer Cells. Mol Imaging Biol 17, 319–327 (2015). https://doi.org/10.1007/s11307-014-0803-7
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DOI: https://doi.org/10.1007/s11307-014-0803-7