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Roseomonas sediminicola sp. nov., isolated from fresh water

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Abstract

A Gram-stain negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated FW-3T was isolated from fresh water and its taxonomic position was investigated by using a polyphasic approach. Strain FW-3T was found to grow at 10–37 °C and at pH 7.0 in the absence of NaCl on nutrient agar. On the basis of 16S rRNA gene sequence similarity, strain FW-3T was shown to belong to the family Acetobacteraceae and to be related to Roseomonas lacus TH-G33T (97.2 % sequence similarity) and Roseomonas terrae DS-48T (96.4 %). The G+C content of the genomic DNA was determined to be 68.0 %. The major menaquinone was determined to be Q-10 and the major fatty acids were identified as summed feature 7 (comprising C18:1 ω9c/ω12t/ω7c as defined by the MIDI system; 55.4 %), and C18:1 2OH (29.8 %). DNA and chemotaxonomic data supported the affiliation of strain FW-3T to the genus Roseomonas. Strain FW-3T could be differentiated genotypically and phenotypically from the recognized species of the genus Roseomonas. The novel isolate therefore represents a novel species, for which the name Roseomonas sediminicola sp. nov. is proposed, with the type strain FW-3T (=KACC 16616T = JCM 18210T).

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Acknowledgments

This work was supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967) and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Korea and by the China Major Projects No.2012ZX09503001-003.

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Authors and Affiliations

  1. KI for the Biocentury, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Dan He, Jin-Kwang Kim, Sun-Chang Kim & Wan-Taek Im

  2. College of Biotechnology, Dalian Polytechnic University, Qinggong-yuan No. 1, Ganjingzi-qu, Dalian, 116034, People’s Republic of China

    Dan He, Xiao-Ye Jiang, Hong-San Yu & Feng Xie Jin

  3. Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Republic of Korea

    Jin-Kwang Kim & Min-Ho Yoon

  4. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 404-708, Republic of Korea

    Hye-Yoon Park

  5. Institute for Brain Disorders, Dalian Medical University, Dalian, 116044, People’s Republic of China

    Changkai Sun

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  1. Dan He
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  2. Jin-Kwang Kim
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  3. Xiao-Ye Jiang
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  7. Min-Ho Yoon
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  8. Sun-Chang Kim
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  9. Feng Xie Jin
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  10. Wan-Taek Im
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Correspondence to Wan-Taek Im.

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He, D., Kim, JK., Jiang, XY. et al. Roseomonas sediminicola sp. nov., isolated from fresh water. Antonie van Leeuwenhoek 105, 191–197 (2014). https://doi.org/10.1007/s10482-013-0065-4

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  • DOI: https://doi.org/10.1007/s10482-013-0065-4

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