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Cloning of Sal1, a scytalone dehydratase gene involved in melanin biosynthesis in Cochliobolus heterostrophus

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Mycoscience

Abstract

A melanin biosynthetic gene, Sal1, in the southern corn leaf blight fungus Cochliobolus heterostrophus, was identified by degenerate and inverse PCR. Structural analysis revealed that the open reading frame is 555 bp in length, interrupted by one intron. Melanin deficiency of the sal1 mutant strain, which accumulates the intermediate scytalone, was successfully complemented by introducing the gene fragment. These results suggest that Sal1 is a scytalone dehydratase gene of C. heterostrophus.

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Author notes

  1. Yoshimoto Saitoh

    Present address: Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry, Chiba, 270-1194, Japan

Authors and Affiliations

  1. Sustainability Research Institute, Tottori University of Environmental Studies, Tottori, 689-1111, Japan

    Yoshimoto Saitoh

  2. Medical Mycology Research Center, Chiba University, Chiba, 260-8673, Japan

    Kiminori Shimizu

  3. Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Kosuke Izumitsu, Atsushi Morita & Chihiro Tanaka

Authors
  1. Yoshimoto Saitoh
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  2. Kosuke Izumitsu
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  3. Atsushi Morita
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  4. Kiminori Shimizu
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  5. Chihiro Tanaka
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Corresponding author

Correspondence to Chihiro Tanaka.

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Saitoh, Y., Izumitsu, K., Morita, A. et al. Cloning of Sal1, a scytalone dehydratase gene involved in melanin biosynthesis in Cochliobolus heterostrophus . Mycoscience 53, 330–334 (2012). https://doi.org/10.1007/s10267-011-0162-z

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  • DOI: https://doi.org/10.1007/s10267-011-0162-z

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