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Advances toward DNA-based identification and phylogeny of North American Armillaria species using elongation factor-1 alpha gene

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Mycoscience

Abstract

The translation elongation factor-1 alpha (EF-1α) gene was used to examine the phylogenetic relationships among 30 previously characterized isolates representing ten North American Armillaria species: A. solidipes (=A. ostoyae), A. gemina, A. calvescens, A. sinapina, A. mellea, A. gallica, A. nabsnona, North American biological species X, A. cepistipes, and A. tabescens. The phylogenetic relationships revealed clear separation of all ten North American Armillaria species, with the exception of one A. gallica isolate that perhaps represents an unnamed cryptic species. These results indicate that the EF-1α gene could potentially serve as a diagnostic tool for distinguishing among currently recognized North American biological species of Armillaria.

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Acknowledgments

The project was supported by USDA Forest Service-RMRS, Forest and Woodland Ecosystems Program, Research Joint Venture Agreement (07-JV-11221662-285), and the Special Technology Development Program of the USDA Forest Service, S&PF Forest Health Monitoring.

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Correspondence to Ned B. Klopfenstein.

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Ross-Davis, A.L., Hanna, J.W., Kim, MS. et al. Advances toward DNA-based identification and phylogeny of North American Armillaria species using elongation factor-1 alpha gene. Mycoscience 53, 161–165 (2012). https://doi.org/10.1007/s10267-011-0148-x

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  • DOI: https://doi.org/10.1007/s10267-011-0148-x

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