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Simple transformation of the rice false smut fungus Villosiclava virens by electroporation of intact conidia

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Mycoscience

Abstract

A simple procedure is reported for transformation of the rice false smut fungus Villosiclava virens (anamorph: Ustilaginoidea virens) using electroporation of intact conidial cells. The transformation vector pCB1004eGFP was constructed with a green fluorescent protein (eGFP) gene under a constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene of Cochliobolus heterostrophus. When a linearized vector was applied, eGFP-expressing transformants were successfully acquired. An inoculation test in rice plants showed that the eGFP-expressing transformants were able to form rice false smut balls.

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Acknowledgments

We thank Dr. T. Ashizawa, R. Sonoda, and Y. Fujita for their advice in relation to the inoculation tests. We also wish to thank Dr. H. Ikegami for his encouragement. This research was supported by Ishikawa Prefectural University.

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Authors and Affiliations

  1. Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa, 921-8836, Japan

    Eiji Tanaka, Tsuyoshi Kumagawa & Hironori Koga

  2. Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Chihiro Tanaka

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  1. Eiji Tanaka
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  2. Tsuyoshi Kumagawa
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  3. Chihiro Tanaka
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  4. Hironori Koga
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Correspondence to Eiji Tanaka.

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Tanaka, E., Kumagawa, T., Tanaka, C. et al. Simple transformation of the rice false smut fungus Villosiclava virens by electroporation of intact conidia. Mycoscience 52, 344–348 (2011). https://doi.org/10.1007/s10267-011-0115-6

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  • DOI: https://doi.org/10.1007/s10267-011-0115-6

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