Abstract
The laccases (EC 1.10.3.2) secreted into solid-state culture by Lentinula edodes were analyzed. The fungus secreted at least two laccases in the solid-state culture. One laccase was purified to a homogeneous preparation using anion-exchange, hydrophobic, and size-exclusion chromatography. SDS-PAGE analysis showed that the purified laccase, Lcc6, was a monomeric protein of 58.5 kDa. The optimum pH for enzyme activity was about 3.5, and the laccase was most active at 40°C. The N-terminal amino acid sequence of Lcc6 did not correspond to the sequence of Lcc1, which was previously purified from L. edodes. Lcc6 had decolorization activity to some chemical dyes.
Similar content being viewed by others
References
Baldrian P (2006) Fungal laccases: occurrence and properties. FEMS Microbiol Rev 30:215–242
Chen S, Ge W, Buswell JA (2004) Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea. Eur J Biochem 271:318–328
Diaz M, Pedregosa AM, De Lucas JR, Torralba S, Monistrol IF, Laborda F (1996) Purification and properties of β-galactosidase from Aspergillus nidulans. Microbiologia 12:585–592
Fukuda T, Uchida H, Takashima Y, Uwajima T, Kawabata T, Suzuki M (2001) Degradation of bisphenol A by purified laccase from Trametes villosa. Biochem Biophys Res Commun 284:704–706
Gold MH, Alic M (1993) Molecular biology of the lignin-degrading basidiomycete Phanerochate chrysosporium. Microbiol Rev 57:605–622
Hoegger PJ, Navarro-González M, Kilaru S, Hoffmann M, Westbrook ED, Kües U (2004) The laccase gene family in Coprinopsis cinerea (Coprinus cinereus). Curr Genet 45:9–18
Iwashita K, Shimoi H, Ito K (2001) Extracellular soluble polysaccharide (ESP) from Aspergillus kawachii improves the stability of extracellular β-glucosidases (EX-1 and EX-2) and is involved in their localization. J Biosci Bioeng 91:134–140
Morisaki K, Fushimi T, Kaneko S, Kusakabe I, Kobayashi H (2001) Screening for phenoloxidases from edible mushrooms. Biosci Biotechnol Biochem 65:2334–2336
Nagai M, Sato T, Watanabe H, Saito K, Kawata M, Enei H (2002) Purification and characterization of an extracellular laccase from the edible mushroom Lentinula edodes, and decolorization of chemically different dyes. Appl Microbiol Biotechnol 60:327–335
Nagai M, Kawata M, Watanabe H, Ogawa M, Saito K, Takezawa T, Kanda K, Sato T (2003) Important role of fungal intracellular laccase for melanin synthesis: purification and characterization of an intracellular laccase from Lentinula edodes fruit body. Microbiology 149:2455–2462
Nagai M, Sato T, Enei H (2004) Bioremediation by laccases from Lentinula edodes. Mushroom Sci 16:573–578
Rios S, Pedregosa AM, Monistrol IF, Laborda F (1993) Purification and molecular properties of an α-galactosidase synthesized and secreted by Aspergillus nidulans. FEMS Microbiol Lett 112:35–41
Schultz A, Jonas U, Hammer E, Schauer F (2001) Dehalogenation of chlorinated hydroxybiphenyls by fungal laccase. Appl Environ Microbiol 67:4377–4381
Terashita T, Inoue T, Nakaie Y, Yoshikawa K, Shishiyama J (1997) Isolation and characterization of extra- and intra-cellular metal proteinases produced in the spawn-running process of Hypsizygus marmoreus. Mycoscience 38:243–245
Tsujiyama S (2003) Effect of vanillin on the production of wood-decomposing enzymes from a wood-rotting fungus, Coriolus versicolor. Mycoscience 44:345–350
Ullah MA, Bedford CT, Evans CS (2000) Reactions of pentachlorophenol with laccase from Coriolus versicolor. Appl Microbiol Biotechnol 53:230–234
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Nagai, M., Sakamoto, Y., Nakade, K. et al. Purification of a novel extracellular laccase from solid-state culture of the edible mushroom Lentinula edodes . Mycoscience 50, 308–312 (2009). https://doi.org/10.1007/s10267-008-0478-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10267-008-0478-5