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Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures

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Abstract

Lignin and lignans share monolignols as common precursors and are both potentially involved in plant defence against pathogens. In this study, we investigated the effects of fungal elicitors on lignin and lignan metabolism in flax (Linum usitatissimum) cell suspensions. Cell suspension cultures of flax were treated with elicitor preparations made from mycelium extracts of Botrytis cinerea, Phoma exigua and Fusarium oxysporum F ssp lini. Elicitors induced a rapid stimulation of the monolignol pathway, as confirmed by the increase in PAL (phenylalanine ammonia-lyase, EC 4.1.3.5), CCR (cinnamoyl-CoA reductase EC 1.2.1.44) and CAD (cinnamyl alcohol dehydrogenase EC 1.1.1.195) gene expression and PAL activity. At the same time, CCR activity only increased significantly in F. oxysporum-treated cells 24 h post elicitation. On the other hand, CAD activity measured for coniferyl alcohol formation was transiently decreased but a substrate-specific activation of CAD activity was observed in F. oxysporum-treated cells when using sinapyl alcohol as substrate. The accumulation of monolignol-derived products varied according to the elicitor used. B. cinerea or P. exigua-elicited cell cultures were characterised by a reinforcement of the cell wall by a deposit of 8-O-4′-linked non-condensed lignin structures and phenolic monomers, while at the same time no stimulation of 8-8′-linked lignan or 8-5′-linked phenylcoumaran lignan accumulation was observed. Additionally, elicitation of cell cultures with F. oxysporum extracts even triggered a strong incorporation of monolignols in the non condensed labile ether-linked lignin fraction concomitantly with a decrease in lignan and phenylcoumaran lignan accumulation. Several hypotheses are proposed to explain the putative role of these compounds in the defence response of flax cells against pathogens.

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Abbreviations

ANHSEC:

Anhydro-secoisolariciresinol

CAD:

Cinnamyl alcohol dehydrogenase

CCR:

Cinnamoyl-CoA reductase;

DCG:

Dehydrodiconiferyl alcohol glucoside

PAL:

Phenylalanine ammonia lyase

PCBER:

Phenylcoumaran benzylic ether reductase

PLR:

Pinoresinol lariciresinol reductase

SAD:

Sinapyl alcohol dehydrogenase

SECO:

Secoisolariciresinol

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Acknowledgments

C. Hano and M. Addi contributed equally to this work. We thank J.-P. Trouvé (coopérative linière Terre de Lin, Fontaine le Dun, France) for gifts of flax seeds cv Barbara, P. exigua and F. oxysporum mycelia, and B. Dehorter (Université des Sciences et Technologies de Lille, France) for giving us B. cinerea mycelium. We thank E. Duverger (Université d’Orléans, France) for epi-fluorescence microscopy. We also wish to thank L. Gutierrez (Université de Picardie Jules Verne, France) for technical advice on RT-PCR experiments. This work was financially supported, in part, by a grant from the “Région Centre” (France) and by the “Conseil Général d’Eure et Loir” (France).

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Authors and Affiliations

  1. Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, 21 rue de Loigny la Bataille, 28000, Chartres, France

    C. Hano, J. Doussot, S. Maury, E. Lainé & F. Lamblin

  2. Laboratoire de Physiologie des Parois Végétales, UPRES EA 3568/USC-INRA, UFR de Biologie Bât SN2, Université des Sciences et Technologies de Lille, 59655, Villeneuve d’Ascq cedex, France

    M. Addi & S. Hawkins

  3. Laboratoire de Phytotechnologie, UPRES EA 3900, Faculté de Pharmacie d’Amiens, Université de Picardie Jules Verne, 1 rue des Louvels, 80037, Amiens cedex 01, France

    L. Bensaddek, S. Baltora-Rosset & F. Mesnard

  4. INRA Parois Végétales et Matériaux Fibreux UMR FARE, 2 esplanade Roland Garros, BP224, 51686, Reims, France

    D. Crônier & B. Chabbert

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  1. C. Hano
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  2. M. Addi
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  3. L. Bensaddek
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  4. D. Crônier
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  5. S. Baltora-Rosset
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  6. J. Doussot
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  7. S. Maury
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  8. F. Mesnard
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  9. B. Chabbert
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  10. S. Hawkins
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  11. E. Lainé
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  12. F. Lamblin
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Correspondence to C. Hano.

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C. Hano and M. Addi contributed equally to this work.

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Hano, C., Addi, M., Bensaddek, L. et al. Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures. Planta 223, 975–989 (2006). https://doi.org/10.1007/s00425-005-0156-1

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