A positive association between oviposition and fruit set: female choice or manipulation?

Abstract 

The relationship between where a female chooses to oviposit and her larvae’s performance at those sites is critical to both the ecology and evolution of plant-insect interactions. For predispersal seed predators that do not themselves pollinate their host and whose larvae are sessile, females must be able to predict which flowers will ultimately be pollinated and set fruit, or be able to manipulate flowers in some way to ensure they set fruit. Otherwise, their offspring will perish. Here we describe the results of an experiment in which we tested if female Hylemya (Diptera: Anthomyiidae) are choosing oviposition sites wisely, or if they are manipulating flowers of their host, Ipomopsis aggregata (Polemoniaceae), in some way to ensure fruit set. Previous work in this system established a positive correlation between oviposition and fruit set. By bagging females on flowers, we removed their ability to choose flowers on which to oviposit. We found that flowers females oviposited on, whether bagged (”no choice”) or unbagged (”female choice”), had a significantly higher probability of setting fruit than ”control” flowers that we bagged but did not cage females on. In addition, we tested if Hylemya prefer particular architectural locations of flowers and if those locations correspond with higher than average fruit set. Although flowers at the distal end of the plant, and those most proximal to the main stem, were more likely to set fruit overall, Hylemya was no more likely to oviposit on those flowers than others on the plant. Taken together, our results suggest that Hylemya is somehow able to manipulate its host to ensure fruit set and thus the provisioning of their larvae.