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R-(+)-verapamil, S-(-)-verapamil, and racemic verapamil inhibit human retinal pigment epithelial cell contraction

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Abstract 

Purpose: To evaluate the effects of R-(+)-verapamil, S-(-)-verapamil, and the racemate on in vitro contraction of human retinal pigment epithelial cells.Methods: RPE cells from human donor eyes were enzymatically dissociated and cultured on collagen gels. Transdifferentiated RPE cells (seventh and eighth passage) were used for experiments. The contraction assays were treated with different concentrations (10−1000 µM) of R-(+)-verapamil, S-(-)-verapamil, and racemic verapamil.  Results: Verapamil inhibited the gel contraction of human RPE cells. R-(+)-verapamil, S-(-)-verapamil, and racemic verapamil acted equally in a dose-dependent manner from 10–1000 µM.Conclusion: The results indicate the effectiveness of verapamil to inhibit the contraction of RPE cells. R-(+)-verapamil is recom-

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Authors and Affiliations

  1. University eye hospital, Liebigstrasse 10–14, 04103 Leipzig, Germany e-mail: faudef@server3.medizin.uni-leipzig.de Tel.: +49–341–9721571, , , , , , DE

    Frank Faude, Volker Enzmann, Enrico Pöschmann, Stefan Hoffmann & Peter Wiedemann

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  1. Frank Faude
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  2. Volker Enzmann
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  3. Enrico Pöschmann
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  4. Stefan Hoffmann
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  5. Peter Wiedemann
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Received: 25 August 1999 Revised: 3 November 1999 Accepted: 4 November 1999

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Faude, F., Enzmann, V., Pöschmann, E. et al. R-(+)-verapamil, S-(-)-verapamil, and racemic verapamil inhibit human retinal pigment epithelial cell contraction. Graefe's Arch Clin Exp Ophthalmol 238, 537–541 (2000). https://doi.org/10.1007/PL00007897

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  • DOI: https://doi.org/10.1007/PL00007897

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